中国病理生理杂志2013，Vol.29Issue(4)：748-751,4.DOI:10.3969/j.issn.1000-4718.2013.04.031
自发性高血压大鼠心肌组织microRNA-133a与TGF-β1蛋白表达的改变及意义
Relationship of microRNA-133a and TGF-β1 protein in myocardial tissues of spontaneously hypertensive rats
摘要
Abstract
AIM: To observe the changes of microRNA-133a and transforming growth factor p, (TGF-β1 ) protein in the myocardium of spontaneously hypertensive rats (SHR). METHODS: Male SHR (18 weeks old, n = 12) and male Wistar-Kyoto rats (WKY, 18 weeks old, n = 12) served as SHR group and control group, respectively. Caudal arterial blood pressure was detected by a noninvasive blood pressure measurement and analysis system. Myocardial collagen volume fraction (CVF) and perivascular collagen area ratio (PVCA) were determined by Masson staining. The level of miR-133a in the heart was detected by real-time quantitative PCR. The protein level of TGF-p, in the heart was also analyzed by the methods of immunohistochemisty and Western blotting. RESULTS: Compared with control group, systolic and diastolic blood pressure, CVF and PVCA significantly increased, the expression of TGF-p, protein was significantly up-regulated, and the level of miR-133a was significantly reduced in SHR group. In SHR group, the expression of miR-133a was decreased to (23. 9 ±4. 6) % in control group. A negative correlation between the levels of miR-133a and TGF-p, protein in SHR group was observed (r= - 0.791, P < 0.01). CONCLUSION: The level of miR-133a is down-regulated along with the up-regulation of TGF-p, protein expression and collagen synthesis in the myocardial tissues of SHR. miR-133a and TGF-β1 may be involved in myocardial fibrosis in SHR.关键词
微小RNA/转化生长因子β1/自发性高血压大鼠/心肌纤维化Key words
MicroRNA/Transforming growth factor β1 /Spontaneously hypertensive rats/Myocardial fibrosis分类
医药卫生引用本文复制引用
谭文鹏,杨侃,陈晞明,陈次滨..自发性高血压大鼠心肌组织microRNA-133a与TGF-β1蛋白表达的改变及意义[J].中国病理生理杂志,2013,29(4):748-751,4.基金项目
广州医学院博士启动基金资助项目(No.2012C59) （No.2012C59）
