中国免疫学杂志2013，Vol.29Issue(3)：260-264,270,6.DOI:10.3969/j.issn.1000-484X.2013.03.009
宫颈癌HeLa细胞内HPV L1蛋白表达的观察
The expression of human papillomaviurs late capsid protein 1 in HeLa cells
摘要
Abstract
Objective: The observations had been done to define the nature of the cytoryctes in Hela cells under electron microscope and to found out the regularity of HPV major capsid protein LI express in HPV positive uterine cervix cancer cells. Methods: HPV LI expression of cultured HeLa cells was detected by ELISA, light microscope and electron microscope immunohistochemistry assays, Western blot assay using HPV LI mice monoclonal antibody. LI mRNA in HeLa cells was detected by reverse transcriptional PCR( RT-PCR) . Results:ELISA assay manifests demonstrated that HPV LI was present in lysate of HeLa cells. HRP labeled light microscope immunohistochemistry showed strong positive reaction in HeLa cells. Observed under electron microscopy, there are lumpish cytorrhyctes materials in the cytoplasm of partial HeLa cells. These cytorrhyctes materials were aggregated by very small and uniform particles and these particles can be marked by HPV LI antibody which was labeled by colloidal gold. Western blot assay present a band at 55 kD district it was LI specific strap which demonstrat HPV18 LI was present in HeLa cells. Conclusion: The cytorrhyctes in HeLa cells under electron microscope are composed of HPV18 LI and LI can be expressed in the HeLa cells.关键词
HeLa细胞/人乳头瘤病毒/宫颈癌/主要外壳蛋白/表达Key words
HeLa cells/ Human papillomavirus/ Cervical cancer/ Major capsid protein/ Expression分类
医药卫生引用本文复制引用
李慧弘,肖长义,袁太宁,许雪梅,黎家华,李红军..宫颈癌HeLa细胞内HPV L1蛋白表达的观察[J].中国免疫学杂志,2013,29(3):260-264,270,6.基金项目
本文为国家自然科学基金资助项目(30770104)和湖北省自然科学基金资助项目(2011CDC002) （30770104）
