中国免疫学杂志2013，Vol.29Issue(3)：265-270,6.DOI:10.3969/j.issn.1000-484X.2013.03.010
细粒棘球蚴囊液对体外培养小鼠T淋巴细胞影响的初步研究
The pilot study on the influence of T-lymphocyte from mouse spleen cells treated with echinococcus granulosus fluid in vitro
摘要
Abstract
Objective:To observe the influence of T-lymphocyte subsets from mouse spleen cells treated with echinococcus granulosus fluid in vitro. Methods: After 72 h co-culture of spleen cells from BABL/c mouse and Hydatid fluid with different concentrations : 100, 200, 300, 500 μl, percentage of CD3 + CD4 + /CD3 + CD8 + T-lymphocyte subsets and CD4 + CD25 + T were detected by FCM, and gene expression of Foxp3 was measured by RT-PCR. The percentage of T-lymphocyte subsets of spleen cells were also detected at different time points (0, 12, 24, 36, 48 h). Results: With the increasing of the concentration of Hydatid fluid, CD4 + T cells to scale down gradually, while the CD8 + T cells ratio slowly rose, leading to the decreased ratio of CD4+ /CD8+. The percentage of CD4 + CD25 + T cells were not rise as the cyst fluid volume increases, but gels peak when in 100 μl Hydatid fluid group. RT-PCR showed Foxp3 mRNA relative expression had the highest value at low concentrations of 100 μl and 200 μl HF group. Conclusion: Echinococcus granulosus cyst fluid can regulate the ratio of CD4VCD8+ inversion, and then, can promote with immunosuppressive effects of percentage of CD4 + CD25 + T cells and Treg cells specific protein-Foxp3 increases in vitro, indicating that echinococcus granulosus may facilitate to evade the host's immune killer.关键词
囊液/T细胞亚群/Treg细胞/Foxp3Key words
Hydatid fluid/T-lymphocyte subsets/Treg cells/Foxp3分类
医药卫生引用本文复制引用
陈小林,印双红,吴向未,陈雪玲..细粒棘球蚴囊液对体外培养小鼠T淋巴细胞影响的初步研究[J].中国免疫学杂志,2013,29(3):265-270,6.基金项目
本文为国家自然科学基金(81060227)和兵团科技支疆项目(2011AB034) （81060227）
