中国免疫学杂志2013，Vol.29Issue(3)：283-287,5.DOI:10.3969/j.issn.1000-484X.2013.03.013
共刺激分子CTLA-4与B7在自身免疫性心肌炎中的作用
The role of costimulatory molecules CTLA-4 and B7 in autoimmune myocarditis
摘要
Abstract
Objective: Study the expression and changes of costimulatory molecule CTLA-4, B7-1 and B7-2 in experimental autoimmune myocarditis ( EAM) and explore the pathogenesis of EAM. Methods: EAM model was established by injecting porcine cardiac myosin into Lewis rats(experimental group, n =30) ,and then the 30 rats were randomly divided into three subgroups in accordance with 14 d, 28 d, 56 d after the first immunization . HE staining was used to observe the change of histopathological characteristics. Im-munohistochemical staining and real-time fluorescence quantitative PCR detection was used to analysis the expression of CTLA-4, CD80, CD86. Results:There were varying degrees of myocarditis change in experimental group; CTLA-4, CD80, CD86 mainly expressed in cardiac cells. After the first immunization, expression of proteins increased gradually from 14 d to 28 d, but decreased in 56 d. Real-time PCR: compared with normal group, the expression of CD80, CD86 mRNA were higher in experimental group ( P < 0. 05 ) . Conclusion: The expression of CD80, CD86 and CTLA-4 promotes heart tissue inflammation, and may closely related to the occurrence and development of EAM.关键词
心肌炎/CTLA-4/CD80/CD86/免疫应答Key words
Myocarditis/ CTLA-4/ CD80/ CD86/ Immune response分类
医药卫生引用本文复制引用
张琰,房光萃,张丽华,杨秀霖,陈敏..共刺激分子CTLA-4与B7在自身免疫性心肌炎中的作用[J].中国免疫学杂志,2013,29(3):283-287,5.基金项目
本文为福建省科技厅资助高校项目(2007F5057)和福建医科大学教授基金项目(JS08008) （2007F5057）
