中国免疫学杂志2013，Vol.29Issue(3)：295-299,5.DOI:10.3969/j.issn.1000-484X.2013.03.016
模式抗原Mannan-TTC的制备及免疫原性分析
Preparation of mode antigen Mannan-TTC and analysis of its immunogenicity
摘要
Abstract
Objective: To preparation of mode antigen Mannan-tetanus toxin fragment C (TTC) and analysis of its immunoge-nicity. Methods: Mannan was conjugated to TTC, thus Mannan-TTC antigen was obtained. The cross-linked product was identificated by SDS-PAGE. The ability to stimulate the proliferation of allogenic T cells by Mannan-TTC-stimulated DCs was detected by mixed lymphocyte reaction (MLR). WST-1 and ELISA were used to analyse the proliferation and IFN-γ secretion in TTC antigen-specific CD4+ T lymphocyte. ELISA was used to detect the levels of TTC-specific antibodies by using immunized mice. Results: Mannan was conjugated to TTC successfully, and mode antigen Mannan-TTC was obtained. Through activation of dendritic cells (DC) , Mannan-TTC could not only increasingly stimulate allogenetic T cells proliferation, but also enhancively stimulate TTC antigen-specific CD4 + T lymphocyte proliferation and secretion of IFN-7. The mice immunization experiments revealed that the Mannan-TTC antigen could stimulate the mouse immune cell produce TTC antigen-specific antibody, but there was no significant difference compared with the control group ( TTC group) . Conclusion: Mode antigen Mannan-TTC was obtained successfully, which can significantly enhance cellular immune responses, and provide a good model antigen for studying antigen presentation and immune responses.关键词
甘露聚糖/免疫原性/模式抗原Key words
Mannan/ Immunogenicity/ Model antigen分类
医药卫生引用本文复制引用
王凡平,王明永,赵东方,马淑君,余文发,杜继英,丁肖华,朱琳琳,谭静..模式抗原Mannan-TTC的制备及免疫原性分析[J].中国免疫学杂志,2013,29(3):295-299,5.基金项目
本文为国家自然科学基金资助项目(31100645)、河南省科技攻关计划项目(102102310154)、河南省高校科技创新人才项目(2012HASTIT024)、河南省高校青年教师骨干项目(2010GGJS-117)、河南省教育厅科学技术研究重点项目(12A320017)资助项目和新乡医学院重点学科开放基金(ZD200928)资助项目 （31100645）
