中国免疫学杂志2013，Vol.29Issue(4)：358-361,365,5.DOI:10.3969/j.issn.1000-484X.2013.04.006
吉非替尼上调NKG2D配体表达增强A549细胞对NK细胞杀伤的敏感性
Geftinib upregulates expression of NKG2D ligands and enhances the susceptibility to NK cell-mediated cytotoxicities in human lung cancer A549 cells
摘要
Abstract
Objective:To explore the effects of gefitinib on the expression of NKG2D ligands in human lung cancer A549 cells and the cytolytic activity of NK cells. Methods: MTT method was used to detect the anti-proliferative ratio of gefitinib on A549 cells. Expression of NKG2D ligands (MICA, MICB, ULBP1, ULBP2, ULBP3) on A549 cells before and after treated with gefitinib, LY294002 (PD-K inhibitor ) , SB203580 (MAPK inhibitor) , STAT21 (STAT3 inhibitor) , Rottlerin (PKC inhibitor) were analysed respectively by flow cytometer. Cytotoxicities of NK cells against A549 cells before and after treated with gefitinib were measured by LDH releasing assay at effect-to-target cell ratios of 5- 1, 10: 1, 20: 1. Results: Expressions of MICB and ULBP1 were increased in A549 cells treated with gefitinib. Effect of MAPK inhibitors ( SB203580) and STAT3 inhibitors ( STAT21) on expressions of NKG2D ligands were not significant, while PD-K inhibitors (LY294002) downregulated expression of MICA. PKC inhibitors (Rottlerin ) increased expression of ULBP1. Cytotoxicity of NK cells against A549 cells treated by gefitinib was significantly enhanced (P <0. 05). Conclusion:This study indicates that gefitinib enhances the susceptibility to NK cell-mediated cytotoxicities by upregulating NKG2D ligands in A549 cells.关键词
肺癌/表皮生长因子受体/自然杀伤细胞/NKG2DKey words
Lung cancer/ Epidermal growth factor receptor/ Natural killer cells/ NKG2D ligands分类
医药卫生引用本文复制引用
梅家转,刘桂举,张晓娟,赵继智,冯睿婷..吉非替尼上调NKG2D配体表达增强A549细胞对NK细胞杀伤的敏感性[J].中国免疫学杂志,2013,29(4):358-361,365,5.基金项目
本文为郑州市科技创新领军人才基金项目(121PLJRC532) （121PLJRC532）
