中国免疫学杂志2013，Vol.29Issue(4)：386-391,6.DOI:10.3969/j.issn.1000-484X.2013.04.012
CXCR1/2受体拮抗剂-G31P对小鼠腹水型肝癌淋巴转移潜能的影响
Effect of CXCR1/2 antagonist on lymphatic metastasis of ascites hepatocarcinoma in mouse
摘要
Abstract
Objective:To establish animal models with murine hepatocarcinoma cell line with high lymphatic metastatic potential HCA-P/L6 and observe CXCR1/2 antagonist-G31P metastatic potential of the model. Methods: The murine hepatocarcinoma cell line with high metastatic lymphatic potential (HCA-P/L6) was subcutaneously inoculated into the medioventral line of mice 615 and the second passage ascites hepatocarcinoma cells were obtained, taken ascites cell count, diluted with normal saline to the concentration of 4 x 107ml~'. Mice were inoculated with the second passage HCA-P/L6 cells in posterior left foot, while subcutaneous injection of G31P or saline as the treated group and control group. Naked eye observed the model in situ mouse the time of footpad tumor appearance and the status of tumor, lymph node enlargement, recorded the health status of mice. On 21" day, dissected the mouse, took lymph node of different parts and measured the longest diameter, and got the in situ transplanted subcutaneous tumor and suspected organs , produced slides for pathology. Excised some lymph nodes for RT-PCR to detect the expression of CXCR2, KC, MIP2 mRNA. Results: The body weight and the volume of in situ transplanted tumor of the groups are different, with significant difference (P<0.05). Spresding rate also has a significant difference. The pathological examination of lymph node of control group showed that heteromorphism is obvious, and multinucleated tumor cells have been widely seen. And only a small part of the visible individually scattered tumor cells in the treated group mice. RT-PCR showed the expression of MIP-2 mRNA of foot pad in situ tumor in the control group mice was significantly increased. Conclusion :CXCRl/2 antagonist-G31P can inhibit the murine hepatocarcinoma lymphatic metastasis.关键词
腹水型肝癌/CXCR1/2/转移Key words
Ascites hepatocarcinoma/ CXCR1/2/ Metastasis分类
医药卫生引用本文复制引用
彭静,王冰,魏晶,王世仪,屈虹,李芳..CXCR1/2受体拮抗剂-G31P对小鼠腹水型肝癌淋巴转移潜能的影响[J].中国免疫学杂志,2013,29(4):386-391,6.基金项目
本文为国家自然科学基金资助项目(No.30772023) （No.30772023）
