中国人兽共患病学报2013，Vol.29Issue(4)：354-360,7.DOI:10.3969/cjz.j.issn.1002-2694.2013.04.008
单增李斯特菌1/2a血清型菌株生物膜形成能力及相关基因差异研究
Biofilm formation of Listeria monocytogenes serotype 1/2a strains and the diversity in correlated genes
摘要
Abstract
To investigate biofilm formation in 5 Listeria monocytogenes serotype l/2a strains and the related gene diversity, a classical 96-cell microtitre plate biofilm production assay using crystal violet staining was employed in this study and scanning electron microscope (SKM) was also used to visualize the formation of biofilm. PCR method was performed to amplify the 12 genes based on full-length primers, and PCR products were sequenced and compared with the reference strain EGD-e from GenBank. Among these 12 genes, 11 were positive in the 5 strains with some bases replacement at some locus of the genes (most of which were synonymous mutations, the others were non-synonymous mutations). However, bapL gene was found in two strains which possess low ability to form biofilm, while the other two strains with high ability of biofilm formation were proved to be negative of bapL gene. The images of biofilm formation of the 5 strains obtained by SEM were consistent with the results of crystal violet microplate assay. Results suggested that the ability to form biofilm were varied in the different Listeria monocytogenes serotype l/2a strains, which also does not directly correlate with bapL gene. In addition, both of the synonymous and non-synonymous mutations are found in the 11 biofilm formation related genes of the 5 strains.关键词
单增李斯特菌/生物膜形成/基因差异Key words
Listeria monocytogenes / biofilm formation/ gene alternation分类
医药卫生引用本文复制引用
叶正兴,王艳,李培京,刘凯,叶长芸..单增李斯特菌1/2a血清型菌株生物膜形成能力及相关基因差异研究[J].中国人兽共患病学报,2013,29(4):354-360,7.基金项目
国家重大传染病防治科技重大专项(2011ZX10004-001)资助 （2011ZX10004-001）
