中国人兽共患病学报2013，Vol.29Issue(4)：369-374,6.DOI:10.3969/cjz.j.issn.1002-2694.2013.04.011
3株人源猪链球菌2型湖南株cps2J、gdh、ef基因进化分析
Phylogenetic analysis of cps2J, gdh and ef genes of 3 human-derived Streptococcus suis serotype 2 strains isolated in Hunan Province
摘要
Abstract
In order to explore the variation and evolutionary status of Streptococcus suis serotype 2 (S. suis 2) isolated in Hunan Province, gene sequences and evolution relationship of cps2], gdh and ef genes of HNCS201001, HNCS201101, and HNCS201102 of human-derived S. suis 2 strains were analyzed. PCR primers of cps2], gdh and ef genes of S. suis 2 were designed, and the genes of HNCS201001, HNCS201101, and HNCS201102 were amplified and sequenced. Then, the nucleotide sequences and the deduced amino acid sequences were analyzed with the Lasergene and Mega 5 software. Analysis of the nucleotide sequences of the cps2J, gdh and ef genes revealed that the length of open reading frames (ORF) were 999 bp, 1 347 bp, and 2 532 bp, coding for 333, 448 and 843 amino acid, respectively. Sequence similarity searches by using the BLAST network service showed HNCS201001, HNCS201101 and HNCS201102 strains belonged to S. suis 2 group, and the nucleotide homolo-gy of cps2], gdh and ef genes among the 3 strains were all 100% ; phylogenetic analysis revealed that the 3 strains in this study and the S. suis 2 strains derived from pigs and human isolated in mainland China and abroad belonged to the same branch. The cps2J, gdh and ef genes of the 3 strains of S. suis 2 isolated in Hunan Province had high homology with other S. suis strains isolated in mainland China and abroad (99. 8%-100% for cps2J gene, 96. 4%-100% for gdh gene, and 99. 7%-100% for ef gene at the nucleotide level; 98. 5%-100% for cps2J gene, 98. 7%-100% for gdh gene, and 99. 5%-100% for ef gene at the a-mino acid level, respectively). It's suggested that the cps2J, gdh and ef genes of the 3 human-derived S. suis 2 strains isolated in Hunan Province have high homology with other S. suis 2 strains isolated in mainland China and abroad, and have a low degree of variation in the gene sequences.关键词
猪链球菌2型/cps2J基因/gdh基因/ef基因/进化分析Key words
Streptococcus suis 2/ cps2J gene/ gdh gene/ef gene/ phylogenetic analysis分类
医药卫生引用本文复制引用
欧新华,张如胜,苏良,杨柳青,肖姗,姚栋,宋克云,孙边成..3株人源猪链球菌2型湖南株cps2J、gdh、ef基因进化分析[J].中国人兽共患病学报,2013,29(4):369-374,6.基金项目
长沙市科技计划项目(K0902167-31) （K0902167-31）
