中国人兽共患病学报2013，Vol.29Issue(4)：412-414,3.DOI:10.3969/cjz.j.issn.1002-2694.2013.04.019
白头翁总皂苷对日本血吸虫糖原、蛋白质及酶的作用
Effect of Pulsatilla chinensis (Bunge) Regel saponins on glycogen, protein and enzymes of Schistosoma japonicum harbored in mice
摘要
Abstract
This article aimed to study the effect of Pulsatilla chinensis (Bunge) Re gel saponins (PRS) on glycogen, protein, and enzymes of Schistosoma japonicum harbored in mice. Mice infected with S. japonicum cercariae for 42 day were treated with PRS at a single dose of 25 mg/kg and were killed 24 h after treatment for collection of worms. The glycogen, protein content, and the activities of AKP, ACP, SOD and GR in female and male worms were measured. Results showed that after the worms were exposed in vivo to 25 mg/kg PRS for 24 h, the glycogen of content of female and male worms significantly decreased by 45. 52% and 23. 2% , respectively. And the protein content of female and male worms significantly decreased by 9. 84% and 10. 66% , respectively. Activities of AKP, ACP, SOD, and GR in female worms were significantly decreased by 43. 5% , 47. 78% , 36. 68% , and 63. 24% , respectively, while in male worms were decreased by 33. 89% , 29. 25% , 17. 15% , and 60. 77% , respectively. In conclusion, PRS showed an apparently inhibitory effect on metabolism of schistosome.关键词
日本血吸虫/白头翁总皂苷/糖原/蛋白质/碱性磷酸酶/酸性磷酸酶/超氧化物歧化酶/谷胱甘肽还原酶Key words
Schistosoma japonicum/ PRS/ glycogen/ protein/ AKP/ ACP/ SOD/ GR分类
医药卫生引用本文复制引用
陈岩勤,诸葛洪祥,许琼明,杨世林,李笑然..白头翁总皂苷对日本血吸虫糖原、蛋白质及酶的作用[J].中国人兽共患病学报,2013,29(4):412-414,3.基金项目
国家自然科学基金(No.81273402),江苏省2011年普通高校研究生科研创新计划项目(CXLX110078) （No.81273402）
