中国蔬菜Issue(14)：31-35,5.
大白菜根肿病抗性基因的标记和定位
Mapping of Clubroot Resistance Gene in Chinese Cabbage
摘要
Abstract
In order to obtain molecular markers closely linked to the clubroot resistance gene in Chinese cabbage [Brassica campestris L.ssp. pekinensis (Lour) Olsson], a segregating F2 population with 197 individuals was constructed by selfing of a Fi hybrid A00645, which is highly resistant to clubroot. Clubroot resistance tests and genetic analysis indicated that the inheritance of clubroot resistance in this material was controlled by a single dominant gene. Based on bulked segregating analysis ( BSA ) and InDel technique, a total of 720 pairs of InDel primers were screened between the resistant and susceptible bulks constructed in F2 population. After further tests among F2 individuals and linkage analysis by JoinMap4.0, 9 InDel markers linked to clubroot resistance gene were obtained. The flanking markers BrID90269 and BrID 11683 closely linked to the resistance gene were located on each side, at genetic distances of 2.0 cM and 2.5 cM, respectively. This clubroot resistance gene was mapped on Scaffold 10, which is in linkage group A8 of Chinese cabbage.关键词
大白菜/根肿病/基因定位/InDel标记Key words
Chinese cabbage/ Clubroot/ Gene mapping/ InDel marker分类
农业科技引用本文复制引用
王彤彤,张淑江,章时蕃,李菲,张慧,孙日飞..大白菜根肿病抗性基因的标记和定位[J].中国蔬菜,2012,(14):31-35,5.基金项目
国家大宗蔬菜产业技术体系(CARS-25-A-01),主要蔬菜作物分子育种与功能基因组研究项目(2012AA100100),农业部园艺作物遗传改良重点开放实验室项目 （CARS-25-A-01）
