中国实验动物学报2013，Vol.21Issue(2)：13-16,4.DOI:10.3969/j.issn.1005-4847.2013.02-003
BABL/c小鼠感染大肠杆菌O127模型的建立及TGF-β1因子的荧光定量检测
Establishment of a mouse model of Escherichia coli O127 infection and real-time PCR detection of TGF-β1 expression
摘要
Abstract
Objective To establish a mouse model of Escherichia coli 0127 infection and to detect the TGF-β1 expression in the colon of mouse models by real-time PCR. Methods Thirty-six BALB/c mice (male: female = 1:1) were used in this study. Colon inflammation in the mice was induced by intragastric administration of Escherichia coli 0127. The expression of TGF-β1 in the colon tissue was analyzed using fluorescent quantitative PCR. Results The mouse models of colon inflammation were successfully established. The TGF-β1 expression level of the experimental group was significantly higher than that of the control group. Conclusions Mouse models of colon inflammation can be successfully established by intragastric gavage of E. coli. Quantitative PCR detection of the TGF-β1 expression can be used as a standard of evaluation of the establishment of the mouse models.关键词
大肠杆菌O127/动物模型/荧光定量/TGF-β1Key words
Escherichia coli/ Mouse model/ Chimeric fluorescent quantitative PCR/ TGF-βl/ Colon inflammation分类
生物科学引用本文复制引用
刘一,隋丽华,曾林,宋宏立,武际荣,陈旖,赵彦斌,刘冰,孙兆增..BABL/c小鼠感染大肠杆菌O127模型的建立及TGF-β1因子的荧光定量检测[J].中国实验动物学报,2013,21(2):13-16,4.基金项目
国家自然科学基金(编号:31272787,31071987). （编号:31272787,31071987）
