中国实验动物学报2013，Vol.21Issue(2)：39-44,后插5,7.DOI:10.3969/j.issn.1005-4847.2013.02.008
线栓法制备不同体重KM小鼠局灶性脑缺血/再灌注模型的建立及评价
Establishment and evaluation of a model of focal cerebral ischemia/reperfusion induced by monofilament in differently weighing Kunming mice
摘要
Abstract
Objective To establish a mouse model of focal cerebral ischemia/reperfusion with arterial monofilament insertion method, and explore the factors affecting the stability of this middle cerebral artery occlusion (MCAO) modeling procedure. Methods One hundred Kunming mice, weighing 20 -40 g, male/female 1 :1, were randomly divided into 10 groups according to the body weight; sham group ( ↑ ) , sham group ( ♀ ) , group A ( ↑ , 20 -24 g) , group B ( ↑ , 25 -29 g) , group C ( ↑ , 30 -34 g) , group D ( ↑ , 35 -39 g) , group E ( ♀ ,20 -24 g) , group F ( ♀ , 25 -29 g) , group G ( ♀ , 30 -34 g) , and group H ( ♀ , 35 -39 g) ,10 mice in each group. Nylon thread (Φ 0. 128 -0. 180 mm) was plugged into the internal carotid artery in mice to occlude the right middle cerebral artery reversibly. The reliability of the MCAO modeling method was evaluated by the degree of neurological deficit scores and 2,3 ,5-triphenyltetrazolium chloride (TTC) staining. Results All groups except the sham groups presented changes in neurobehavioral function and distinct infarction area in TTC staining after cerebral ischemic reperfusion. There were significant increases of behavioral scores and infarct volumes in the groups A and C (P <0. 01), compared with that in the groups C and D. The neurological deficit scores in the groups E and F were markedly higher than that in the groups G and H ( P < 0. 01 ). Meanwhile, the volume of cerebral infarction was significantly increased in the groups E and F in comparison with that in the groups G and H ( P < 0. 05) . Compared with the female mice, the success rates of MCAO models were higher in male mice while the mortality rates were lower. The neurological scores in the group A were significantly higher than that in the group E (P < 0. 01). In addition, there were significant increases of behavioral score and infarct volume in the group B ( P < 0. 01 ) , compared with that in the group F. Conclusions The establishment method of mouse model of focal cerebral ischeraic/ reperfusion with arterial nylon monofilament insertion is stable and simple. However, there are many key factors affecting the success of this method, such as appropriately matching the mouse body weight with the nylon monofilament diameter, the mouse strain and gender selection, depth of thread insertion, operation time, and suitable temperature ( ca. 26℃ ) during experiment. Experimental investigation shows that male Kunming mice weighing about 25 g are most suitable for making focal cerebral ischemic/reperfusion models and serve relevant researches.关键词
小鼠/局灶性脑缺血/再灌注模型/线栓法/体重Key words
Mice/ Focal cerebral ischemic/reperfusion model/ Arterial thread insertion method/ Body weight分类
生物科学引用本文复制引用
甄毅岚,王亚男,李晟,董金龙,盛汝祥,史开宇,董六一..线栓法制备不同体重KM小鼠局灶性脑缺血/再灌注模型的建立及评价[J].中国实验动物学报,2013,21(2):39-44,后插5,7.基金项目
安徽省高校自然基金重点项目(KJ2012A155). （KJ2012A155）
