中国实验动物学报2013，Vol.21Issue(2)：72-74,3.DOI:10.3969/j.issn.1005-4847.2013.02.015
H9N2亚型猪流感病毒诱导小鼠急性肺损伤中TNF-α、IL-1β、IL-6和IL-10的变化和作用
Changes and roles of TNF-α, IL-1β, IL-6 and IL-10 in acute lung injury induced by H9N2 subtype swine influenza virus in mice
摘要
Abstract
Objective To explore the changes and roles of inflammatory factors in acute lung injury induced by swine influenza virus H9N2 in mice. Methods Eighty 6-8 week old female Balb/c mice were divided into experimental and control groups (n =40). The mice of experimental group were infected with swine influenza virus (H9N2) by nose dropping. Lung tissue specimens of the mice were taken on day 2, 4, 6, 10 and 14 after infection, and the concentrations of TNF-α, IL-1β, IL-6 and IL-10 in the tissue homogenate were determined. Results Concentrations of TNF-a, IL-1β, IL-6 and IL-10 in the lung tissue homogenate of infected mice were significantly higher than that in the control group at different time points (P < 0. 05 ) . TNF-α and IL-1β were tended towards normal, but IL-6 and IL-10 were still at high levels after 14 days. Conclusions Inflammatory factors play a major role in acute lung injury induced by H9N2 subtype swine influenza virus in mice. TNF-a and IL-1β display a proinflammatory effect, while IL-10 is likely to play an anti-inflammatory role, similar to that of IL-6.关键词
H9N2亚型猪流感病毒/BALB/c小鼠/急性肺损伤/炎症因子Key words
Swine influenza virus (H9N2) / BALB/c mice/ Acute lung injury/ Inflammatory factor分类
生物科学引用本文复制引用
魏东,刘英,贾宁,徐彤,刘贵河..H9N2亚型猪流感病毒诱导小鼠急性肺损伤中TNF-α、IL-1β、IL-6和IL-10的变化和作用[J].中国实验动物学报,2013,21(2):72-74,3.基金项目
河北省自然基金(C2009001028和C2011405002). （C2009001028和C2011405002）
