中国药房2013，Vol.24Issue(15)：1386-1388,3.DOI:10.6039/j.issn.1001-0408.2013.15.15
UPLC法同时测定不同产地知母药材中新芒果苷和芒果苷的含量
Simultaneous Determination of Neomangiferin and Mangiferin in Anemarrhena asphodeloides from Different Habitats by UPLC
摘要
Abstract
OBJECTIVE: To establish a method for simultaneous determination of neomangiferin and mangiferin in Anemarrhe-na asphodeloides and determine the contents of two components in A. asphodeloides from different habitats, and to evaluate the quality of A. asphodeloides from different habitats. METHODS: UPLC method was adopted. The determination was performed on ACQUITY UPLC BEH C18(100 mm×2.1 mm, 1.7 μm) column with mobile phase consisted of 0.4% acetic acid-methanol (gradient elution) at the flow rate of 0.25 ml/min. The column temperature was 35 ℃ and the detection wavelength was set at 258 nm. RESULTS: The linear ranges of neomangiferin and mangifer were 0.030 0-0.480 0 μg and 0.028 5-0.456 0 μg(r=0.999 9).RSDs of precision test,reproducibility test and stability test were all lower than 3%. The average recoveries were 97.67% (RSD=2.87% , n=6) and 100.17%(RSD=0.78% ,n=6), respectively. CONCLUSION: The method is rapid and accurate, and can be used for simultaneous determination of neomangiferin and mangiferin in A. asphodeloides. The qualities of A asphodeloides from different habitats are different.关键词
知母/超高效液相色谱法/芒果苷/新芒果苷/不同产地/含量测定Key words
Anemarrhena asphodeloides/ UPLC/ Mangiferin/ Neomangiferin/ Habitat/ Content determination分类
医药卫生引用本文复制引用
钟可,王文全,靳凤云,侯俊玲,刘进涛,赵志刚..UPLC法同时测定不同产地知母药材中新芒果苷和芒果苷的含量[J].中国药房,2013,24(15):1386-1388,3.基金项目
国家公益性(中医药)行业科研专项资助项目(No.201107009-03) （中医药）
