中国药房2013，Vol.24Issue(15)：1406-1408,3.DOI:10.6039/j.issn.1001-0408.2013.15.22
改良RP-HPLC法同时测定牡丹皮药材中芍药苷和丹皮酚的含量
Simultaneous Determination of Paeoniflorin and Paeonol in Paeonia suffruticosa by Modified RP-HPLC
摘要
Abstract
OBJECTIVE: To provide more effective means for the quality control of Paeonia suffruticosa by improving the method for simultaneous determination of paeoniflorin and paeonol in P. suffruticosa. METHODS: Agilent ZORBAX Eclipse XDB C18 (250 mm×4.6 mm,5 μm) column was used with mobile phase consisted of methanol-water (gradient elution) at the flow rate of 1.0 ml/min. The detection wavelength was 230 nm for paeoniflorin and 274 nm for paeonol. RESULTS: The linear range of paeoniflorin was 0.060 33-1.206 6 μg and that of paeonol was 0.200 4-4.008 0 μg(r=0.999 9). RSDs of precision test,reproducibility test and stability test were all lower than 2%. The average recoveries were 99.49% and 98.66% with RSDs of 2.0% and 1.8% respectively (n=6). CONCLUSION: The modified method removes phosphate corrosion for chromatographic column. The method is simple, rapid and accurate, andean be used for the qualitative analysis of paeoniflorin and paeonol in P. suffruticosa and its preparations.关键词
牡丹皮/芍药苷/丹皮酚/反相高效液相色谱法/改良/含量测定Key words
Paeonia suffruticosa/ Paeoniflorin/ Paeonol/ PR-HPLC/ Modified/ Content determination分类
医药卫生引用本文复制引用
邓开英,朱必越,朱照静..改良RP-HPLC法同时测定牡丹皮药材中芍药苷和丹皮酚的含量[J].中国药房,2013,24(15):1406-1408,3.
