全反式维甲酸对人肝癌SMMC-7721细胞侵袭能力的影响OA北大核心CSCDCSTPCD
All-trans Retinoic Acid Inhibits Invasion of Human Hepatocellular Carcinoma SMMC-7721 Cells
目的 探讨全反式维甲酸(ATRA)对人肝癌SMMC-7721细胞迁移和侵袭的影响及其分子机制.方法 划痕愈合和侵袭试验检测ATRA对SMMC-7721细胞迁移与侵袭能力的影响；Real-time PCR、Western blot检测ATRA作用SMMC-7721细胞后CD147、MMP-2的表达.结果 (1)划痕试验结果显示,以10、20、40 μmol· L-1 ATRA处理SMMC-7721细胞24 h后,较未处理组划痕距离明显增加(P＜0.…查看全部>>
Objective To investigate the effects of all-trans retinoic acid (ATRA) on the migration and invasion of human hepatocellular carcinoma SMMC-7721 cells and its molecular mechanism. Methods The effects of ATRA on migration and invasion were examined by scratch healing assay and Transwell assay,and the expressions of mRNA and proteins of CD147 and MMP-2 in treated SMMC-7721 cells were determined by real-time PCR and Western blot respectively. Results Scratch he…查看全部>>
李海燕;曹励民;王秉亮
医药卫生
全反式维甲酸肝癌侵袭
all-trans retinoic acid hepatocellular carcinoma invasion
《中国医科大学学报》 2013 (2)
106-110,5
陕西省卫生厅科研基金资助项目(2012D75)陕西省教育厅科学研究项目计划(12JK0703)西安交通大学医学院校级科研基金资助项目(2006QN5)
评论