中国医科大学学报2013，Vol.42Issue(2)：149-151,3.
GST-HDAC4融合蛋白载体的构建及其蛋白表达
Construction of GST-HDAC4 Fusion Protein Vector and Its Protein Expression
摘要
Abstract
Objective To construct the prokaryotic expression vector of human HDAC4 protein and glutathione-S-transferase (GST) for protein expression and purification. Methods pcDNA3.1-HDAC4 was digested by EcoR I ,the hHDAC4 coding sequence was obtained and cloned into prokaryotic expression vector pGEX-5X-1 to generate novel vector GST-HDAC4. After identification of GST-HDAC4, Escherichia coli BL21 was transformed and induced by isopropyl-β-D-thiogalactoside (IPTG),and target protein was obtained after purification. The expression of the recombinant plasmid was proved by Western blot. Results Restriction enzyme digestion and sequencing indicated that prokaryotic expression vector of GST-HDAC4 fusion protein was successfully constructed. Coomassie brilliant blue staining and Western blot revealed that GST-HDAC4 fusion protein with bioactivity was successfully obtained. Conclusion The prokaryotic expression vector of HDAC4 was successfully constructed. The fusion protein of GST-HDAC4 with bioactivity has been obtained.关键词
GST-HDAC4/原核表达/融合蛋白Key words
GST-HDAC4/ prokaryotic expression/ fusion protein分类
生物科学引用本文复制引用
邵阳光,刘姣,李丰..GST-HDAC4融合蛋白载体的构建及其蛋白表达[J].中国医科大学学报,2013,42(2):149-151,3.基金项目
国家自然科学基金资助项目(30900752,31271389) （30900752,31271389）
