中国医科大学学报2013，Vol.42Issue(4)：293-295,300,4.
高压氧对大鼠脊髓损伤后Bcl-2表达的影响
Effect of Hyperbaric Oxygen on Bcl-2 Expression after Spinal Cord Injury in Rats
摘要
Abstract
Objective To investigate the effect and significance of hyperbaric oxygen (HBO) on expression of Bcl-2 protein after spinal cord injury in rats. Methods Fifty-four female adult Wistar rats, weighing between 250 and 300 g.were randomly divided into sham operation group,spinal cord injured group and experimental group. The spinal cord injury was induced by Allen's method. Rats in experimental group were treated with HBO,while the rats in other two groups were rearing under conventional condition. Animals were sacrificed at different time points and tissues were collected. The expression of Bcl-2 protein and the change of spinal cord histology were detected by immuno-chemistry and hematoxylin-eosin (HE) staining. Results The HE staining results of histology showed structural disorder of spinal cord, hemorrhage,edema,and a number of vacuolation in injured spinal cord;the above mentioned symptoms were completely relieved in HBO group but not in sham operation group. The immunochemishy results showed only a few cells in sham operation group were positive for Bcl-2, which was widely visible in injured group and experimental group; significantly increased number of Bcl-2-positive cells were observed in experimental group compared with the spinal cord injured group (P < 0.05). Conclusion The HBO inhibits the apoptosis of neuron by increasing the expression of Bcl-2 protein, and thus play a therapeutic role for spinal cord injury.关键词
高压氧/脊髓损伤/Bcl-2Key words
hyperbaric oxygen/ spinal cord injury/ Bcl-2分类
医药卫生引用本文复制引用
王岩峰,秦光华,杨明超,王喆,李雷..高压氧对大鼠脊髓损伤后Bcl-2表达的影响[J].中国医科大学学报,2013,42(4):293-295,300,4.基金项目
国家自然科学基金资助项目(81100924) （81100924）
教育部高校博士学科点专项科研基金项目(20092104120006) （20092104120006）
辽宁省教育厅高校科研计划(L2010687) （L2010687）
辽宁省博士启动科研基金资助项目(20081049) （20081049）
