中国医科大学学报2013，Vol.42Issue(4)：321-325,5.
舒芬太尼后处理对缺血再灌注大鼠肌钙蛋白Ⅰ的影响
Effect of Sufentanil Postconditioning on Cardiac Troponin Ⅰ Induced by Myocardial Ischemia-reperfusion Injury in Rats
摘要
Abstract
Objective To investigate the effect of sufentanil postconditioning on cardiac troponin I induced by myocardial ischemia-reperfu-sion injury in rats and cardioprotection. Methods Male Sprague-Dawley rats [body weight 250 (32 g) ] were randomly divided into six groups (n =72):Sham (n =12): the coronary suture was passed,not tied,and maintained for 150 min; I/R (n =12):ischemia for 30 min followed by 120 min reperfusion; Post (n =12): three cycles of ischemic postconditioning (30 s of ischemia and 30s of reperfusion) at the onset of reperfusion following 30 min of ischemia;Sufen (LS,MS,HS,n =12 respectively):respectively administration of 0.75,3 and 15 μg·kg-1 sufentanil for 3 min at the onset of reperfusion following 30 min of ischemia. Heart rate and mean arterial pressures were measured at the following time points:end of stabilization prior to ischemia (baseline);30 min of ischemia;30,60,90,and 120 min of reperfusion;at the end of 120 min reperfusion,the rats were sacrificed for assessment of cTnI,infarct area,cell morphology of light and electron microscopy. Results No significant differences existed in heart rate between groups in all time points (P > 0.05), mean arterial pressures were significantly decreased at 60,90,and 120 min following reperfusion (P < 0.05) in I/R and at 90 and 120 min following reperfusion (P < 0.05) in the LS group compared to Sham;cTnI and infarct area in Post,MS and HS group were significantly decreased compared to I/R group;in cell morphology of light and electron microscopy, the damage of myocardial structure was significantly reduced in Post, MS and HS group. Conclusion Sufentanil postconditioning could induce cTnI and protect myocardium against I/R injury.关键词
舒芬太尼/后处理/心肌缺血再灌注损/肌钙蛋白ⅠKey words
sufentanil/ postconditioning/ myocardial reperfusion injury/ troponin Ⅰ分类
医药卫生引用本文复制引用
沈途,陈晓光,吴巧玲,张锦英..舒芬太尼后处理对缺血再灌注大鼠肌钙蛋白Ⅰ的影响[J].中国医科大学学报,2013,42(4):321-325,5.基金项目
辽宁省教育厅高校科研计划(2009A727) （2009A727）
