中国医科大学学报2013，Vol.42Issue(4)：339-343,5.
白藜芦醇对脂多糖诱导的H9c2心肌细胞损伤的影响
The Effect of Resveratrol on Lipopolysaccharide-induced Impairment of H9c2 Cardiac Myoblasts
刘玲 1刘新伟 1梁灿鑫 1何东伟 1俞莹 1王萍1
作者信息
摘要
Abstract
Objective To investigate the effect of resveratrol on lipopolysaccharide-induced impairment of H9c2 cardiac myoblasts and its relevance to Sirt1 expression. Methods After cultured for 24 h, H9c2 cells were randomly divided into 4 groups: control group (CON), resveratrol-treated control group (RES),model group (LPS) and resveratrol- and lipopolysaccharide-treated group (RL). Cells in group RES and RL were treated with resveratrol for 24 h while cells in group CON and LPS were treated with vehicle at equal volume. After that,cells in group LPS and RL were treated with lipopolysaccharide for 24 h while cells in group CON and RES were treated with vehicle at equal volume. Lactate dehydrogenase in the culture was determined with Lactate Dehydrogenase Detecting Assay Kit. Malondialdehyde was detected by means of reacting with thiobarbituric acid. Reactive oxygen species in cells was detected with Fluorescence Probe Labeling Reactive Oxygen Species Assay Kit. The expression of Sirtl was determined with Western blot. Results Compared with in group CON, lactate dehydrogenase release, malondialdehyde and reactive oxygen species content in group LPS were substantially increased while Sirtl expression was markedly decreased. Compared with in group LPS,lactate dehydrogenase release,malondialdehyde and reactive oxygen species content in group RL were remarkably decreased, accompanied by Sirt1 expression being significantly increased. Conclusion Resveratrol attenuates lipopolysaccharide-induced oxidative injury in H9c2 cardiac myoblasts, which is probably mediated by resveratrol-induced improvement of Sirt1 expression in lipopolysaccharide-stimulated cells.关键词
白藜芦醇/脂多糖/H9c2心肌细胞/活性氧/Sirt1Key words
resveratrol/ lipopolysaccharide/ H9c2 cardiac myoblasts/ reactive oxygen species/ Sirt1分类
医药卫生引用本文复制引用
刘玲,刘新伟,梁灿鑫,何东伟,俞莹,王萍..白藜芦醇对脂多糖诱导的H9c2心肌细胞损伤的影响[J].中国医科大学学报,2013,42(4):339-343,5.
