中国中医药信息杂志2013，Vol.20Issue(2)：50-52,3.DOI:10.3969/j.issn.1005-5304.2013.02.017
和胃安神方对失眠大鼠脑组织谷氨酸与γ-氨基丁酸含量的影响
Effect of Hewei Anshen Decoction on Cerebral Contents of Glutamic Acid and Gamma-aminobutyric Acid in Insomnic Rats
摘要
Abstract
Objective To study the effect of Hewei Anshen Decoction on cerebral contents of glutamic acid (GLU) and garnma-aminobutyric acid (GABA) in insomnic rats and explore its mechanism in treating insomnia. Method Forty healthy male Wistar rats were randomly divided into normal group, model group, Hewei Anshen Decoction group and diazepam group, with 10 rats in each group. All rats but those in the normal group were intraperitoneally injected with PCPA to make model of insomnia. Hewei Anshen Decoction group were treated with Hewei Anshen Decoction, diazepam group with diazepam solution, and the other two groups with normal saline for 6 days. After that, rats were killed, hippocampus and hypothalamus were removed and the contents of GLU and GABA were analyzed using high performance liquid chromatography (HPLC). Result Compared with normal group, GLU levels in hippocampus and hypothalamus of model group were significantly higher, while GABA levels significantly lower (P<0.01). Compared with model group, GLU levels in hippocampus and hypothalamus of Hewei Anshen Decoction group were significantly lower, and GABA levels significantly higher (P<0.01). Conclusion Hewei Anshen Decoction can treat insomnia through regulating central neurotransmitters GLU and GABA of insomnic rats.关键词
和胃安神方/失眠/谷氨酸/γ-氨基丁酸/海马/下丘脑/大鼠Key words
Hewei Anshen Decoction/ insomnia/ GLU/ GABA/ hippocampus/ hypothalamus/ rats分类
医药卫生引用本文复制引用
黎发根,李绍旦,杨明会,周建平..和胃安神方对失眠大鼠脑组织谷氨酸与γ-氨基丁酸含量的影响[J].中国中医药信息杂志,2013,20(2):50-52,3.基金项目
国家自然科学基金(81173429) （81173429）
首都医学发展基金(SF-2009-Ⅱ-16) （SF-2009-Ⅱ-16）
