安徽农业科学2013，Vol.41Issue(6)：2477-2479,3.
牛传染性鼻气管炎病毒的分离鉴定及gD基因序列分析
Isolation, Identification and Sequence Analysis of gD Gene of Infectious Bovine Rhinotracheitis Virus
杨有武1
作者信息
- 1. 青海省海北藏族自治州海晏县兽医站,青海海晏812200
- 折叠
摘要
Abstract
[Objective] The research aimed to isolate a strain of virus from nasal swab samples of cattle which might be infected by infectious bovine rhinotracheitis(IBR) and make the identification and sequencing analysis. [Method] A strain of virus was isolated from nasal swab samples of cattle which might be infected by infectious bovine rhinotracheitis( IBR) and it was named as NM strain. The isolated strain was i-dentified by PCR, MDBK cell lesion observation, neutralization test, animal regression test and gD gene sequence analysis. [ Result] The isolated strain was confirmed as virulent strain of infectious bovine rhinotracheitis virus. NM strain made MDBK cell to produce typical cell lesion of infectious bovine rhinotracheitis virus. TCID50 of NM strain determined by Reed-Muench method was 10-6.0/ml. NM strain could make neutralization test with IBRV positive serum, but it didn't react with IBRV negative serum. IBRV antibody-free calves with the age of 8 months showed typical clinical symptoms of IBR after inoculation of NM strain. The sequencing analysis results showed that the nucleotide homology between NM strain and K22 strain of IBRV. [ Conclusion] The research laid the foundation for developing the diagnosis reagent and its vaccine of IBR.关键词
牛传染性鼻气管炎病毒/分离/序列/鉴定Key words
Infectious bovine rhinotracheitis virus/Isolation/Sequence/Identification分类
农业科技引用本文复制引用
杨有武..牛传染性鼻气管炎病毒的分离鉴定及gD基因序列分析[J].安徽农业科学,2013,41(6):2477-2479,3.
