王璐 1解晓雯 1王佳 1张馨桐 1辛元昌 1任辉1
作者信息
摘要
Abstract
[Objective] To study waste beer yeast intensification autolysis and optimize intensification factors. [ Method ] Single-factor test was conducted on SOD, amino acid, glucose and SOD activity in waste beer yeast after the release; and physical factors orthogonal test was designed in order to optimize parameters. Adding glucanase, papain into above active substances to promote autolysis process, chemical factors orthogonal test was designed to optimize parameters. [Result] The results showed that the optimal combination of amino acid extraction is pH 5.0, water amount 300% , salt 3% ; the optimal combination of glucose content is pH 5. 0, water amount 250% , salt 1 % ; the optimal combination of SOD content is pH 5.5 , water amount 300% , salt 2% , the optimal combination of SOD activity is pH 5. 5, water amount 250% , salt 1%. The biochemical factors orthogonal test showed that, the optimal combination of amino acid is pH 6. 0, temperature 60℃, enzyme dosage 6 g/kg glucanase +4 g/kg papain, the optimal combination of glucose content is pH 6.0, temperature 55℃, enzyme dosage 6 g/kg glucanase +4 g/kg papain, the optimal combination of SOD content is pH 6. 0, temperature 55℃, enzyme dosage 8 g/kg glucanase +4 g/kg papain, the optimal combination of SOD activity is pH 6.5, temperature 60℃, enzyme dosage 6 g/kg glucanase + 4 g/kg papain. [ Conclusion] Under the optimal combination conditions, it could be simplify operation process, decrease operation difficulties, improve utilization rate and yield of yeast.关键词
啤酒废酵母/自溶条件/优化参数Key words
Waste beer yeast/Autolysis condition/Optimization parameters分类
农业科技
