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DNA电化学传感器中电极修饰条件的探讨

赵燕珍 王捷 张海燕 吴小丽

北京生物医学工程2013,Vol.32Issue(2):201-205,5.
北京生物医学工程2013,Vol.32Issue(2):201-205,5.DOI:10.3969/j.issn.1002-3208.2013.02.19.

DNA电化学传感器中电极修饰条件的探讨

Modified conditions of DNA electrochemical sensor

赵燕珍 1王捷 1张海燕 1吴小丽1

作者信息

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摘要

Abstract

Objective To investigate the best preparation condition of ssDNA single-layer film. Methods A DNA modified gold electrode was obtained with the technology of self-assembled monolayer( SAM). The concentration of DNA and the time of modification were studied by cyclic voltammetric technique. Results The results showed that a SAM was formed on the surface of gold electrode. The best SAM could be obtained in 80 μmol/L capture fixed for 12 h. Conclusions The generation of sulfur gold key could form a stabilized SAM in gold electrodes.

关键词

DNA电化学传感器/自组装/金电极/循环伏安法

Key words

DNA electrochemical sensor/ self-assembly/ gold elect rode/ cyclic voltammetry

分类

医药卫生

引用本文复制引用

赵燕珍,王捷,张海燕,吴小丽..DNA电化学传感器中电极修饰条件的探讨[J].北京生物医学工程,2013,32(2):201-205,5.

基金项目

传感技术联合国家重点实验室基金(skt1003)、广东省自然科学基金博士启动项目(10451001002004935)资助 (skt1003)

北京生物医学工程

OACSTPCD

1002-3208

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