动物营养学报2013,Vol.25Issue(3):643-650,8.DOI:10.3969/j.issn.1006-267x.2013.03.024
方斑东风螺饲料中锌需要量的研究
Dietary Zinc Requirement of Spotted Babylon, Babylonia areolate
摘要
Abstract
This experiment was conducted to determine the dietary zinc (Zn) requirement of spotted babylon, Babylonia areolate. Six doses of 0, 5, 10, 20, 30 and 50 mg/kg Zn from zinc sulfate were supplemented in a basal diet (casein and gelatin were used as protein sources) to prepare 6 experimental diets. In the 6 experimental diets, the Zn levels were 7.1, 10.1, 15. 3, 23. 4, 33. 0 and 54.4 mg/kg, respectively. Each diet was fed one group (each group had 3 replicates and each replicate had 80 spotted babylon) of spotted babylon with an initial body weight of (106. 25 ± 0. 00) mg for 8 weeks. The Zn content in rearing seawater was 5. 74 μg/L. The results showed as follows; the survival rate (SR), feed conversion rate (FCR), protein efficiency rate (PER) and soft body to shell ratio (SB/SR) were not significantly affected by dietary Zn content (P >0. 05). With the increase of dietary Zn content, the weight gain rate (WGR) was significantly increased firstly (P <0. 05) , and then there was no significant difference among all groups when dietary Zn content reached 15. 3 mg/kg (P >0. 05). 2) No significant differences were found in the contents of moisture, crude protein, ether extract and ash in soft body of spotted babylon among all groups (P >0. 05). 3) The soft body Zn content of spotted babylon had a positive correlation with dietary Zn content (P <0. 05, R2 =0. 975 0) , but the shell Zn content was not significantly affected by dietary Zn content (P >0. 05). 4) With the increase of dietary Zn content, the activities of total superoxide dismutase (T-SOD) , copper-zinc superoxide dismutase ( CuZn-SOD) , catalase (CAT) and alkaline phosphatase (AKP) in visceral mass of spotted babylon were significantly increased firstly (P < 0. 05) , and then no significant differences were found in above enzymic activities among all groups when dietary Zn content reached 23. 4 mg/kg (P >0. 05). Using the broken-line model with WGR as the dependent variable, the optimum dietary Zn requirement of spotted babylon is estimated to be 15. 3 mg/kg. Analysis by broken-line of the activities of T-SOD, CuZn-SOD, CAT and AKP in visceral mass indicates that the optimum requirement of juvenile spotted babylon for dietary Zn is 22. 9 to 24. 3 mg/kg.关键词
方斑东风螺/锌/需要量Key words
spotted babylon/ zinc/ requirement分类
农业科技引用本文复制引用
杨原志,吴业阳,董晓慧,谭北平,杨奇慧,迟淑艳,刘泓宇..方斑东风螺饲料中锌需要量的研究[J].动物营养学报,2013,25(3):643-650,8.基金项目
广东省自然科学基金(10152408801000024) (10152408801000024)
广东省科技计划项目(2009B020309008) (2009B020309008)
广东省高等职业院校珠江学者岗位计划资助项目(2011) (2011)