南方医科大学学报2013，Vol.33Issue(3)：322-325,331,5.DOI:10.3969/j.issn.1673-4254.2013.03.03
山楂酸预处理对皮层神经元氧糖剥夺损伤的影响
Protective effect of maslinic acid preconditioning against oxygen-glucose deprivation-induced injuries in embryonic rat cortical neurons
摘要
Abstract
Objective To investigate the effect of maslinic acid preconditioning against injuries of rat cortical neurons induced by oxygen-glucose deprivation (OGD). Methods The cortical neurons were isolated from Sprague-Dawley rat embryos at 15-17 days of gestation for primary culture. The cortical neurons were incubated with different concentrations (0.1, 1, and 10 μmol/L) of maslinic acid prior to OGD. The cell damage and viability were evaluated for lactate dehydrogenase (LDH) leakage and using MTT assay, respectively, and the expression of Bax protein was detected using Western blotting. Results OGD significantly increased LDH release rate and decreased the viability of the cells. After preconditioning with maslinic acid (1 and 10 umol/L), LDH leakage rate was decreased and cell the viability increased in cells exposed to OGD. Western blotting showed that Bax expression in the cells decreased as maslinic acid concentrations increased. Conclusion Pretreatment with maslinic acid can protect cultured embryonic rat cortical neurons against OGD-induced injury possibly in relation to decreased expression of Bax.关键词
山楂酸/神经元/原代培养/氧糖剥夺Key words
maslinic acid/ cortical neurons/ primary culture/ oxygen-glucose deprivation引用本文复制引用
黄柏胜,罗洁,韩仰,李思敏,刘发益,邬力祥..山楂酸预处理对皮层神经元氧糖剥夺损伤的影响[J].南方医科大学学报,2013,33(3):322-325,331,5.基金项目
国家自然科学基金(30770838,81202515) （30770838,81202515）
湖南省重点学科建设项目 （）
