南方医科大学学报2013，Vol.33Issue(3)：351-355,5.DOI:10.3969/j.issn.1673-4254.2013.03.09
蛋白激酶D1在烟曲霉介导的NF-κB信号通路激活及转录活性中的作用
Functional role of protein kinase D1 in Aspergillums fumigates-induced activation of nuclear factor-κB signal pathway and transcription
摘要
Abstract
Objective To explore the functional role of protein kinase D1 (PKD1) in the activation of nuclear factor-κB (NF-κB) signal pathway and NF-κB transcription mediated by Aspergillums fumigates. Methods A549 cells and HEK293 cells were transfected with green fluorescence protein (GFP) or GFP-PKD1 followed by treatment with 1×105 CFU/ml Aspergillus fumigatus conidia for different time lengths. The phosphorylation levels of PKD1, IKB and p65 (pS276) in the transfected cells were measured by Western blotting. A549 cells were transfected with GFP-PKD1 or siRNA-PKD1, and the phosphorylation of IKB and p65 (pS276) was examined. Finally, NF-κB-1uc and renilla luciferase reporter pRL-SV40 were cotransfected into GFP- or GFP-PKD1-transfected A549 cells before exposure of the cells to Aspergillus fumigatus conidia for 24 h, and NF-κB transcriptional activity in the cells was determined using dual-luciferase reporter assay. Results Overexpression of PKD1 significantly increased Aspergillus fumigatus conidia-stimulated phosphorylation of PKD1,IKB and p65 (pS276), whereas PKD1 knockdown by siRNA-PKD1 suppressed IKB and p65 (pS276) phosphorylation. Dual luciferase assay demonstrated that PKD1 overexpression markedly enhanced Aspergillus fumigatus-induced NF-κB transcription in A549 cells. Conclusion PKD1 may contribute to the activation of NF-κB signal pathway and NF-κB transcription induced by Aspergillums fumigates.关键词
蛋白激酶D1/烟曲霉分生孢子/NF-κB/转录激活/磷酸化Key words
protein kinase D1/ Aspergillus fumigatus conidia/ nuclear factor-κB/ transcriptional activity/ phosphorylation引用本文复制引用
牛晓璐,许万福,李松玉,柯志勇,曾方银,罗深秋,邓凡..蛋白激酶D1在烟曲霉介导的NF-κB信号通路激活及转录活性中的作用[J].南方医科大学学报,2013,33(3):351-355,5.基金项目
国家自然科学基金(30973014,81272852) （30973014,81272852）
广东省自然科学基金(10151051501000040) （10151051501000040）
广东省自然科学基金博士启动基金(9451051501003704) （9451051501003704）
