南方医科大学学报2013，Vol.33Issue(3)：386-390,5.DOI:10.3969/j.issn.1673-4254.2013.03.16
高糖环境对肝星状细胞TLR4表达及脂多糖诱导的炎症因子表达的影响
Effect of high glucose on toll-like receptor 4 expression and LPS-induced proinflammatory cytokine production in hepatic stellate cells in vitro
摘要
Abstract
Objective To investigate the effect of high glucose on the expressions of toll-like receptor 4 (TLR4) and proinflammatory cytokine production induced by lipopolysaccharide (LPS) in hepatic stellate cells in vitro. Methods Hepatic stellate cell line T6 was cultured in vitro and stimulated by high glucose. The mRNA and protein expression of TLR4 were detected by RT-PCR and Western blotting, respectively. After a 24-h pretreatment with high or low glucose, the cells were stimulated with LPS for 2 h, and Western blotting was used to detect the nuclear translocation of nuclear factor-KB (NF-κB); at 24 h of LPS exposure, the cells were examined for MCP-1 and IL-6 mRNA and protein expression levels with RT-PCR and ELISA, respectively. Results High glucose significantly increased the mRNA and protein expressions of TLR4 (P<0.01) in a time- and dose-dependent manner. High glucose promoted NF-κB nuclear translocation and significantly enhanced the expression and secretion of both MCP-1 and IL-6 (P<0.01). Pretreatment with high glucose significantly promoted LPS-induced NF-κB nuclear translocation (P<0.01) and the mRNA expression and secretion of MCP-1 and IL-6. Conclusions High glucose can increase TLR4 mRNA and protein expressions in hepatic stellate cells and promote LPS-induced NF-κB activation and production of proinflammatory cytokines.关键词
高糖/肝星状细胞/Toll样受体4Key words
high glucose/ hepatic stellate cells/ Toll-like receptor 4引用本文复制引用
史海涛,董蕾,刘亚萍,贾淼,赵刚,赵菊辉,鲁晓岚..高糖环境对肝星状细胞TLR4表达及脂多糖诱导的炎症因子表达的影响[J].南方医科大学学报,2013,33(3):386-390,5.基金项目
西安交通大学医学院第二附属医院重大科研方向资助计划[YJ(ZDJH)201102] （ZDJH）
