肝胆胰外科杂志2013，Vol.25Issue(2)：91-95,5.
肝硬化门静脉高压症患者肠系膜微动脉收缩反应变化及RhoA/ROCK通路的研究
Changes of superior mesenteric arteriole on the contractile response and RhoA/ROCK pathway mechanism in PHT with cirrhosis
摘要
Abstract
Objective To study the changes of PHT superior mesenteric arteriole on the contractile response to norepinephrine with cirrhosis caused by hepatitis B. The mechanism of RhoA/ROCK pathway mechanism in PHT was also explored. Methods Clinical patients were chosen who received operations because of PHT with cirrhosis caused by hepatitis B and non-PHT patients due to other benign diseases at the Department of General Surgery and Transplantation Surgery in our hospital from January 2010 to June 2011. Non-PHT group contains 8 cases, 4 cases of common bile duct stones, and 4 cases of choledochal cyst; 7 cases were of PHT with cirrhosis caused by hepatitis B. Intraoperative measurement of portal pressure was measured. Took the third level intestinal mesentery vessels with their mesentery next to the jejunum and studied the contractile response to norepinephrine of the intestinal mesenteric arterioles. RhoA/ROCK pathway associated proteins' expression and activity changes were measured by Western blotting method. Results The dose-response curve of isolated mesenteric arterioles to norepinephrine from PHT patients with cirrhosis shifted to the right, and the EC50 value was higher than that of non-PHT patients (P<0.01). Compared with that of non-PHT patients, the protein of RhoA in mesenteric arteries of PHT patients with cirrhosis did not change significantly (P>0.05), but the protein express and activity of ROCK-1 were significantly reduced (P<0.01). Conclusion superior mesenteric arteriole on the contractile response to vasocontrictive agents decrease in PHT with cirrhosis, and the impaired RhoA/ROCK pathway is involved in this reaction.关键词
高血压,门静脉/肝硬化/肌源性反应/RhoA/ROCK通路Key words
hypertension, portal/ liver cirrhosis/ myogenic response/ RhoA/ROCK pathway分类
医药卫生引用本文复制引用
秦骏,吴志勇,陈炜,刘德军,罗蒙..肝硬化门静脉高压症患者肠系膜微动脉收缩反应变化及RhoA/ROCK通路的研究[J].肝胆胰外科杂志,2013,25(2):91-95,5.基金项目
国家自然科学基金项目(30972920). （30972920）
