广东医学2013，Vol.34Issue(7)：994-998,5.
Cdx2-RNA基因沉默转染胃癌MGC-803细胞对中药人参黄芪复方药物敏感性的影响
Effects of transfected Cdx2-RNA gene silencing on drug sensitivity of compound traditional Chinese medicine of Ginseng and Astragalus in human gastric cancer MGC-803 cells
摘要
Abstract
Objective To investigate the effect of transfected Cdx2 gene silencing of recombinant lentivims particles ( Cdx2 - RNAi - LV ) on drug sensitivity of traditional Chinese medicine ( TCM ) compound of Ginseng and Astragalus in human gastric cancer MGC - 803 cells. Methods The expression of Cdx2 gene was assessed by reverse transcription polymerase chain reaction ( RT - PCR ) and Western blotting; the cell proliferation was assessed by MTT assay; the cell apoptosis was observed by flow cytometry; the cell colony of formation was detected by Giemsa staining; and the cell migration was assessed by cell scratch experiment. Results Cdx2 gene was successfully silenced by transfected Cdx2 gene silencing recombinant lentivims particles in human gastric cancer MGC - 803 cells. The TCM compound of Ginseng and Astragalus could inhibit the cellular growth, inhibit the colony formation, induce cell apoptosis, reduce cell migration of the human gastric cancer cell line MGC -8031; which was significantly promoted by Cdx2 silencing when comparing with those using TCM compound alone ( P < 0. 05 ). Conclusion Cdx2 gene silencing increases the the drug sensitivity of TCM compound of Ginseng and Astragalus in gastric cancer cells.关键词
中药人参黄芪复方/Cdx2/胃癌/药物敏感性Key words
traditional chinese medicine compound of Ginseng and Astragalus/ Cdx2/ gastric cancer/ drug sensitivity引用本文复制引用
韦尉元,吴琨,王晓通,谢玉波,肖强..Cdx2-RNA基因沉默转染胃癌MGC-803细胞对中药人参黄芪复方药物敏感性的影响[J].广东医学,2013,34(7):994-998,5.基金项目
国家自然科学基金资助项目(编号:81060201),广西自然科学基金资助项目(编号:2011GXNSFA018273),广西科学研究与技术开发计划项目(编号:桂科攻1298003-2-6),广西壮族自治区中医药管理局中医药科技专项课题(编号:GZKZ09-71) （编号:81060201）
