桂林理工大学学报2012，Vol.32Issue(4)：610-612,3.DOI:10.3969/j.issn.1674-9057.2012.04.030
偶氮胭脂红B共振散射光谱法测定硫酸庆大霉素
Determination of Gentamycin Sulfate with Azocarmine B by Resonance Light Scattering Spectrometry
摘要
Abstract
In Britton-Robinson buffer solution of pH 2. 8, when azocarmine B reacts with gentamycin sulfate (GEN) to form association complex, the resonance light scattering signal (RLS) of system can be enhanced. The maximum scattering peak is 285 nm and the enhanced RLS intensity is proportional to the concentration of GEN. Based on that, a new method for the determination of GEN is developed. The linear range is 0. 02 ~ 3. 5 μg/mL and the detection limit is 0. 012 μg/mL. The application of determination of GEN in injection samples provides satisfactory result.关键词
硫酸庆大霉素/偶氮胭脂红B/共振散射光谱法Key words
gentamycin sulfate/azocarmine B/resonance light scattering spectrometry分类
化学化工引用本文复制引用
唐宁莉,凌悦菲,蒙华毅,李曾奎..偶氮胭脂红B共振散射光谱法测定硫酸庆大霉素[J].桂林理工大学学报,2012,32(4):610-612,3.基金项目
国家自然科学基金项目(21165008) （21165008）
