湖南农业大学学报（自然科学版）2013，Vol.39Issue(2)：166-172,封3,8.DOI:10.3724/SP.J.1238.2013.00166
鱼腥草快速繁殖技术体系的建立
Established the rapid propagation technological system of Houttuynia cordata Thunb
摘要
Abstract
The subterranean stems of Houttuynia cordata Thunb were employed for the purpose of establishing the rapid propagation system using explants sterilization, adventitious bud differentiation and multiplication, rooting and transplanting approaches in the paper. Results from the research were as follows: CD The best sterilization approach was to sterilize explants using streptomycin and penicillin (0.5 mg/L) for 12 hours firstly, then using HgCl2 (0.1%) for 12 minutes and HgCl2 (0.1%) for 10 minutes respectively; (2) The best condition for the germination and growth of adventitious buds was adding 0.5 mg/L NAA and 2.0 mg/L 6-BA to MS medium, while, if 0.5 mg/L NAA and 1.0 mg/L 6-BA were added to MS medium, the propagation of adventitious buds could be effectively promoted. (3) Adding 4-5 mg/L GA3 to MS medium could promote proliferation and growth of adventitious buds; (4) NAA had a good promotional effect on improve budding and rooting of Houttuynia cordata Thunb. The optimal transplanting medium was cultivated soil and vermiculite which could result in 99% survival rate and healthy growing regeneration seedlings.关键词
鱼腥草/组织培养/快速繁殖Key words
Houttuynia Cordata Thunb/ tissue culture/ rapid propagation分类
农业科技引用本文复制引用
黄放,李炎林,钟军,熊兴耀..鱼腥草快速繁殖技术体系的建立[J].湖南农业大学学报（自然科学版）,2013,39(2):166-172,封3,8.基金项目
科技部国家基础条件平台建设基金(2004DKA30430) （2004DKA30430）
湖南农业大学人才科学基金(2003YJ007) （2003YJ007）
