湖南农业大学学报（自然科学版）2013，Vol.39Issue(2)：183-187,5.DOI:10.3724/SP.J.1238.2013.00183
猪血清中抗猪丹毒丝菌特异性IgG的纯化
Purification of Erysipelothrix rhusiopathiae specific IgG from swine serum
摘要
Abstract
To obtain Erysipelothrix rhusiopathiae(E. rhusiopathiae) special IgG with high specificity and purity, we use the antigen reverse adsorption combined with recombination protein A (SPA) of staphylococcus affinity chromatography to purify the specific IgG from swine serum. The result showed that the volume of serum showed little influence on the amount of IgG obtained, while the amount of bacteria was positively related to the yield of IgG E.rysipelothrix rhusiopathiae specific IgG was first purified from swine serum, and then subjected to SPA affinity chromatography, the final concentration of the IgG obtained is 240 μg/mL, thus, we get 360 μg antibody from the 8 mL swine serums, the purity of which is about 100% which can be applied in phage display.关键词
猪丹毒丝毒/特异性免疫球蛋白G(IgG)/猪血清/IgG纯化/纯度/重组葡萄球菌蛋白A凝胶(SPA)/SDS-聚丙烯酰胺凝胶电泳(SDSPAGE)Key words
Erysipelothrix rhusiopathiae/ specific immunoglubelin G(IgG)/ swine serum/ IgG purification/ purity/staphylococcal protein A(SPA)/ sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)分类
农业科技引用本文复制引用
胡云霏,钱幸,王淑琴,余兴龙,刘晓波,罗璋..猪血清中抗猪丹毒丝菌特异性IgG的纯化[J].湖南农业大学学报（自然科学版）,2013,39(2):183-187,5.基金项目
湖南省科学技术厅重点项目(2011FJ2017) （2011FJ2017）
