解剖学杂志2013，Vol.36Issue(1)：17-20,4.DOI:10.3969/j.issn.1001-1633.2013.01.005
迷迭香酸对大鼠肾结石形成和间α胰蛋白酶抑制物表达的影响
Effect of rosmarinic acid on renal stone formation and expression of inter-alpha-trypsin inhibitor in rat
摘要
Abstract
Objective: To investigate the effect of rosmarinic acid on renal stone formation and the expression of inter-alpha-trypsin inhibitor (IαI) in rat. Methods: The model control rats with calcium oxalate renal stone formation were induced by ethylene glycol and ammonium chloride for 28 days. And, different doses of rosmarinic acid were applied as the interference factor, and Shenshitong Keli as positive control drug. The contents of urine oxalic acid and Ca2+ and the blood biochemistry after 28d of rats in each group were detected Von-Kossa's staining was employed to observe renal calcium oxalate crystal morphology distribution and the renal pathological changes. Computer image analysis was carried out to evaluate the immunohis-tochemical expression of IαI in the rat kidney tissues. Results: There were significant differences in the expression of IαI in the kidney tubules epithelial cells, the oxalic acid, Ca2+ content in urine and BUN, UA, Cr content between the rosmarinic acid middle-dose (0. 05 g/kg) and high-dose groups (0. 1 g/kg) and the renal stone control group. They both had difference in calcium oxalate crystal morphology distribution compared to the renal stone group. Conclusion: Rosmarinic acid can inhibit calcium oxalate renal stone formation and reduce the expression of IαI in rats.关键词
迷迭香酸/间α胰蛋白酶抑制物/肾结石Key words
rosmarinic acid/ inter-alpha-trypsin inhibitor/ renal stone引用本文复制引用
黄幼霞,黄荣桂,郑兴中..迷迭香酸对大鼠肾结石形成和间α胰蛋白酶抑制物表达的影响[J].解剖学杂志,2013,36(1):17-20,4.
