解剖学杂志2013，Vol.36Issue(1)：25-28,4.DOI:10.3969/j.issn.1001-1633.2013.01.007
淫羊藿苷对糖尿病大鼠睾丸中亚硝基谷胱甘肽还原酶及Bcl-2的影响
Effect of icariin on s-nitrosoglutathione reductase and Bcl-2 in diabetic rats testis
摘要
Abstract
Objective :To observe the effect of icariin on s-nitrosoglutathione reductase (GSNOR) and Bcl-2 in diabetic rats testis. Methods: Sprague-Dawley rats were induced by an injection of streptozotocin. Four weeks after injection of STZ, the rats were randomly divided into 5 groups: a normal group, a control group, a low dose group (30 mg/kg, ig), a middle dose group (70 mg/kg, ig) and a high dose group (100 mg/kg, ig). The blood of the heart was tested for testosterone and estradiol. Im-munohistochemical technique was used to detect the expression of GSNOR and Bcl-2. Results: Compared with control group, the ratio of testosterone and estradiol in the middle dose group were increased, and the fasting food glucose was descend evidently between the control group and the middle dose group. Numerous pathological changes (decreased spermatogenic cell, distortion seminiferous tubule) were lessening in diabetic rats after receiving ICA. Immunohistochemistry showed that there were higher GSNOR protein expressions in the low dose group and middle dose group. A high expression of Bcl-2 protein was observed in Leydig cells. Significant differences existed in the control group, the low dose group and the middle dose group. Conclusion: Icariin evidently relieves testicular damage in diabetic rats by improving the ratio of testosterone and estradiol,reducing the level of FFG and increased the expression of GSNOR and Bcl-2 protein.关键词
亚硝基谷胱甘肽还原酶/B细胞淋巴瘤/白血病-2基因/淫羊藿苷/糖尿病/睾丸/免疫组织化学Key words
s-nitrosoglutathione reductase/ Bcl-2/ icariin/ diabetes/ testis/ immunohistochemistry引用本文复制引用
李丹,魏莎莉,谢怡,陈渝,刘祖翠,刘龙浩..淫羊藿苷对糖尿病大鼠睾丸中亚硝基谷胱甘肽还原酶及Bcl-2的影响[J].解剖学杂志,2013,36(1):25-28,4.基金项目
重庆市科学技术委员会自然科学基金(CSTC,2010BB4104) （CSTC,2010BB4104）
