实用临床医药杂志2013，Vol.17Issue(7)：8-11,4.DOI:10.7619/jcmp.201307003
肿瘤抑素对婴幼儿血管瘤内皮细胞增殖活力与细胞周期的影响
Effect of tumstatin on proliferation and cell cycle of endothelial cell in infantile hemangioma
侯团结 1刘漪沦 1李平松 1黄金华 1施泽宏 1马乐 1陈啸1
作者信息
摘要
Abstract
Objective To explore the influence of anti-angiogenic drugs tumstatin on proliferation in vitro and cell cycle of endothelial cell in infantile hemangioma. Methods Hemangioma was cut in vitro digestion to obtain endothelial cells in infantile hemangioma which were cultured in vitro and passaged. Different concentrations of tumstatin peptide were added to the culture medium. MTT assay was applied to detect cell viability, and flow cytometry was employed to detect the changes of cell cycle. Results Following induction of fetal bovine serum (FBS) of 2. 5% volume fraction, tumstatin inhibited the proliferation activity of endothelial cells, showing a dose-effect relationship. Under the influence of 17 μg/mL concentration of tumstatin, the cell cycle of endothelial cells in G0/ G1 phase was 55.8% , while the it was 42.9% in the control group. Conclusion Tumstatin can effectively inhibit the in vitro proliferation activity of the endothelial cells of infantile hemangioma, and show some dose-effect relationship. Tumstatin can affect cell cycle progression, prompting their arrest in the G0/G1 phase.关键词
肿瘤抑素/婴幼儿血管瘤/内皮细胞Key words
tumstatin/ infantile hemangioma/ endothelial cell分类
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侯团结,刘漪沦,李平松,黄金华,施泽宏,马乐,陈啸..肿瘤抑素对婴幼儿血管瘤内皮细胞增殖活力与细胞周期的影响[J].实用临床医药杂志,2013,17(7):8-11,4.
