实用临床医药杂志2013，Vol.17Issue(7)：16-19,4.DOI:10.7619/jcmp.201307005
慢性乙型肝炎患者肝细胞内HBsAg、HBcAg的表达与肝组织病理、血清HBV DNA定量关系
Correlation among the expression of HBsAg,HBcAg in hepatocytes and pathological changes in liver tissues, quantity of serum HBV DNA in patients with chronic hepatitis B
摘要
Abstract
Objective To study the correlation among the expression of HBsAg, HBcAg in hepatocytes and the pathology changes in liver tissue, the quantity of HBV DNA in serum and in the liver tissue in patients with chronic hepatitis B. Methods Expression of HBsAg and HBcAg in hepatocytes was assayed by using immuohistochemical staining. HE staining and immuohistochemical staining were used to identify the extent of liver inflammation and fibrosis and the quantity of HBV DNA in serum and liver tissue. At the time the pathological diagnosis was performed liver biopsy was performed in 188 CHB patients. Results The positive expression rates of HBsAg and HBcAg in liver tissue in CHB patients were 95.7% and 77.1 % , respectively. 98.3% of HBsAg expression was cytoplasm type, 62.1 % of HBcAg expression was cytoplasm membrance type and 37.9 % was nucleus type. The degree of liver inflammation amd fiborsis were both more serious in cytoplasm membrance type compared with nucleus type (Mann-whitney U = 1966. 500. 000, Wilcoxon W = 3506.500 P= 0.028 P<0.01; Mann-whitney U = 2005.500, Wilcoxon W = 3445.500 P = 0.045 P<0. 05). The quantity of HBV DNA in serum and liver were significantly higher in cytoplasm membrance type compared with nucleus type. There existed statistical differences(x2 = 8.214, P = 0.042; x2 = 11.250, P = 0.010). Conclusion The results suggest that HBcAg expression type is correlated with HBV replication, and liver inflammation and fibrosis in the immunopathogenesis of liver damage in CHB. HBcAg might be a reliable index to evaluate antivirus treatment.关键词
慢性乙型肝炎/乙型肝炎病毒DNA/表面抗原/核心抗原/炎症/纤维化/免疫组织化学Key words
chronic hepatitis B/ HBV DNA/ HBsAg/ HBcAg/ inflammation/ fibrosis/immunohistochemistry分类
医药卫生引用本文复制引用
刘爱平,叶军,耿爱文..慢性乙型肝炎患者肝细胞内HBsAg、HBcAg的表达与肝组织病理、血清HBV DNA定量关系[J].实用临床医药杂志,2013,17(7):16-19,4.
