军事医学2013，Vol.37Issue(4)：241-245,5.DOI:10.7644/j.issn.1674-9960.2013.04.001
肿瘤细胞中Ro60的表达及其对肿瘤细胞辐射敏感性的影响
Ro60 overexpression contributes to proliferation and sensitivity of tumor cells to γ-irradiation
摘要
Abstract
Objective To investigate the expression and subcellular localization of RNA -binding protein Ro60 in neoplasms before and after γ-ray irradiation , and the function of Ro60 in tumor cell proliferation and radio-sensitivity. Methods The eukaryotic expression plasmid of mGFP -Ro60 was constructed and transfected into HCT116 and MCF7 tumor cells. The cellular localization of Ro60 was examined before and after irradiation. Cell proliferation and radio -sensitivity were detected by CCK8 and trypan blue assay. Results The result of immunoblotting showed that tumor cells expressed Ro 60 protein. The irradiation increased Ro60 expression and induced significantl nuclear aggregation of Ro 60. The cell proliferation before and after irradiation was drastically reduced while cell death increased in Ro 60 overexpressed tumor cells. Conclusion γ-ray irradiation alters Ro60 expression and localization , and Ro60 plays an important role in tumor cell proliferation and ra -dio-sensitivity.关键词
Ro60/肿瘤/细胞增殖/辐射敏感性Key words
Ro60/ neoplasm / cell proliferation / radio-sensitivity分类
医药卫生引用本文复制引用
楼敏铭,张兴,田宝磊,刘继来,刘斌,张令强,郑晓飞,宋宜,孙志贤..肿瘤细胞中Ro60的表达及其对肿瘤细胞辐射敏感性的影响[J].军事医学,2013,37(4):241-245,5.基金项目
国家自然科学基金资助项目(30900167) （30900167）
