军事医学2013，Vol.37Issue(4)：274-278,5.DOI:10.7644/j.issn.1674-9960.2013.04.009
芋螺多肽Eb1.6的制备工艺研究
Preparation method of conopeptide Eb1.6
摘要
Abstract
Objective To probe a method for large -scale synthesis of α-conopeptide Eb 1. 6 in order to provide a large quantity of α-conopeptide Eb1. 6 for preclinical trial. Methods Starting with Rink resins and Fmoc -protected amino acids , linear Ebl. 6 was synthesized by solid-phase peptide synthesis method (SPPS) in which DIC/HOBt was used as a coupling agent. The linear peptide (0. 2 mg/ml) was folded in 0. 1 mol/L NH4HCO3 buffer at room temperature for 16 to 24 h. The folded peptide was then absorbed by the Amberlite XAD16 resin,and desorbed by alcohol. Finally, the crude Ebl. 6 was concentrated by rotary evaporation and purified by reversed -phase HPLC. Results The crude yield of linear peptide was 94. 6% ,and the recovery rate of folded Ebl. 6 by Amberlite XAD16 was 82. 3% . Ebl. 6 with a purity of more than 98. 5% was obtained after the purification of RP-HPLC. Conclusion The purity and yield of Ebl. 6 are high when DIC/HOBt is used as a coupling agent. The absorption of Eb 1. 6 with Amberlite XAD16 resin and desorption by alcohol are a highly effi -cient method for concentration of folded Eb 1.6 at a low cost.关键词
α-芋螺多肽/Eb1.6/固相合成/折叠/纯化Key words
α-conopeptide/ Ebl. 6/ solid-phase peptide synthesis method / folding/ purification分类
化学化工引用本文复制引用
徐艳,余硕,姬泓巍,董铭心,王孝花,吴巧玲,戴秋云..芋螺多肽Eb1.6的制备工艺研究[J].军事医学,2013,37(4):274-278,5.基金项目
海洋863项目(2011AA09070108) （2011AA09070108）
国家973计划资助项目(2010CB529802) （2010CB529802）
