江西农业学报2013，Vol.25Issue(4)：52-54,62,4.
金线莲组培快繁技术体系的研究
Study on Tissue Culture and Rapid Propagation Techniques of Anoectochilus roxburghii
摘要
Abstract
Different tests were conducted to optimize the tissue culture conditions of Anoectochilus roxburghii. The results showed that 75% ethanol ( soaking for 30 seconds) + 0.1% HgCl ( soaking for 8 minutes) had better disinfection effect on the ex-plants. The best medium for the bud inducement was MS + BA 2.0 mg/L + NAA 0.3 mg/L, while the best medium for the bud proliferation was MS + BA 1.5 mg/L + NAA 0.5 mg/L. Addition of 20% banana puree in the proliferation medium could improve bud proliferation and culture of strong seedlings. In the culture process of bud differentiation and the culture medium of MS + BA 0. 05 mg/L + NAA 0. 5 mg/L, the inoculum density of 10 plants per bottle and the light treatment of 800 lx could produce ideal effects.关键词
金线莲/茎段/组培快繁/芽丛生/芽诱导Key words
Anoectochilus roxburghii/ Stem segment/ Tissue culture and rapid propagation/ Bud proliferation/ Bud inducement分类
农业科技引用本文复制引用
李艳冬,叶红霞,陈丽萍..金线莲组培快繁技术体系的研究[J].江西农业学报,2013,25(4):52-54,62,4.基金项目
浙江省公益技术研究农业项目"药用植物金线莲种苗规模化生产技术研究"(2010C32108) （2010C32108）
浙江省湖州市一般科研项目"药用植物金线莲规模化生产技术研究"(2009YN02). （2009YN02）
