临床神经病学杂志2013，Vol.26Issue(2)：118-121,4.
吡格列酮对脑缺血再灌注损伤大鼠的神经保护作用及其机制的研究
Investigation on neuroprotection of Pioglitazone and its mechanisms in rats with cerebral ischemia reperfusion injury
摘要
Abstract
Objective To investigate the neuroprotection of Pioglitazone and its mechanisms in rats with cerebral ischemia reperfusion (CIR) injury. Methods Eighty SD rats were randomly divided into sham-operation group (group A) , CIR model group ( group B) , Pioglitazone group ( group C) , GW9662 + Pioglitazone group (group D) and Dimethyl sulfoxide group (group E) , with 16 rats in each group. Rats model of CIR injury were established by suture embolism, relevant medicines (normal saline in group A and group B)were intravenous injected into rats 3 d before operation respectively, one time a day for 3 d. After 24 h of reperfusion, neurological deficit score ( NDS) were performed for each group, ELISA was respectively used to detect levels of NF-KB and IFN-γ in cerebral tissue, HE staining was used to observe morphologic changes of neuron cells in ischemia region, Nissl and TUNEL staining were used to detect the number of neurons and neuron apoptosis. Then the linear correlation analysis was used to determine the association between the levels of NF-KB and IFN-γ in cerebral tissue ( group B, group C). Results Compared with group A, the NDS, the levels of NF-KB and IFN-γ, the number of neurocytes apoptosis were significantly increased and the number of neurons was significantly decreased in the other four groups ( P < 0.05 —0.01) ; compared with group C, the NDS, the levels of NF-KB and IFN-γ and the number of neurocytes apoptosis were significantly increased and the number of neurons was significantly decreased in group B, D and E ( all P < 0. 05) ; the levels of IFN-γ and NF-KB in group B and C were perfect positive correlations ( r = 0. 952, r = 0. 978, all P < 0. 001). Conclusion Pioglitazone provides significantly protection from CIR injury. It is probably through to down-regulating NF-kB levels and then to reduce the release of IFN-γ, and increase the number of neurons and inhibit the apoptosis of neurons, thus protecting the neurons.关键词
脑缺血再灌注/吡格列酮/炎症因子/神经保护作用/细胞凋亡Key words
cerebral ischemia reperfusion/Pioglitazone/inflammatory cytokines/neuroprotection/cell apoptosis分类
医药卫生引用本文复制引用
余智,刘开祥,廖小明,唐永刚,齐立,孙军..吡格列酮对脑缺血再灌注损伤大鼠的神经保护作用及其机制的研究[J].临床神经病学杂志,2013,26(2):118-121,4.基金项目
广西自然科学基金(2012GXNSFAA053102) （2012GXNSFAA053102）
桂林市科技局第一批科学研究与技术开发项目(20110119-1-6) （20110119-1-6）
