环境与职业医学2013，Vol.30Issue(4)：274-277,4.
温州市部分公共场所集中空调冷却水军团菌污染状况
Legionella Pollution in Cooling Water of Central Air Conditioning Systems in Public Places of Wenzhou
摘要
Abstract
[ Objective ] To investigate Legionella pollution in cooling water of central air conditioning system in public places of Wenzhou. [ Methods ] A total of 126 water samples of cooling towers were collected from 30 public places (hospitals, hotels, and supermarkets) of Wenzhou in 2010 and 2011. Isolated pathogen cultivation and fluorescent quantitative polymerase chain reaction (PCR) were used to detect Legionella organisms in the water samples. [ Results ] In 2010 and 2011, the positive rates of Legionella were 62.50% and 33.93%, respectively. Of the 56 strains isolated, 33 were identified as Legionella pneumophila Lp1-15, of which 19.64% was Lp6 (11/56) and 17.86% was Lpl (10/56) and both were predominant serological types and principally derived from hospitals. According to the results of fluorescent quantitative PCR detection, positive 16S rRNA genes were found in 56 Legionella isolates, of which 35 isolates showed mip gene positive, accounting for 62.50%. [ Conclusion ] Legionella pollution is commonly found in the cooling water of central air conditioning systems in Wenzhou public places, and Lp6 and Lpl are predominant serological types.关键词
军团菌/空调冷却水/mip基因Key words
Legionella/ air conditioning cooling water/ mip gene分类
医药卫生引用本文复制引用
章乐怡,洪程基,李毅,郑文力,马雪莲..温州市部分公共场所集中空调冷却水军团菌污染状况[J].环境与职业医学,2013,30(4):274-277,4.基金项目
温州市2010科技计划项目(编号:Y20100022) （编号:Y20100022）
