林业科学2013，Vol.49Issue(2)：33-38,6.DOI:10.11707/j.1001-7488.20130206
植物磺肽素在杂交鹅掌楸体胚发生中的作用
Effects of Phytosulfokine on the Somatic Embryogenesis of Liriodendron hybrids (L.chinense × L.tulipifera)
摘要
Abstract
Phytosulfokine ( PSK ) , a new type of plant growth regulator, has multiple biological activities besides promoting single cell proliferation. In this paper, we imvestigated the role of PSK-α during somatic embryogenesis of Liriodendron hybrids(L. chinense ×L. tulipifera) using immature zygotic embryos as explants. The result showed that PSK-α was able to improve cell division and proliferation and induce embryogenic cells formation. At the early stage of somatic embryogenesis, 0. 1 mg·L-1 PSK-α increased the ratio of callus formation from explants and improved cell state; 0.5 mg-L PSK-α induced embryogenic callus formation; and 0.8 mg·L-1 PSK-α was in favor of somatic embryo development and growth of regeneration plantlets.关键词
杂交鹅掌楸/体细胞胚胎发生/胚性愈伤组织/再生植株/植物磺肽素Key words
Liriodendron hybrids/ somatic embryogenesis/ embryogenic callus/ plantlet regeneration/ PSK分类
农业科技引用本文复制引用
陈金慧,张艳娟,吴亚云,王鹏凯,王光萍,施季森..植物磺肽素在杂交鹅掌楸体胚发生中的作用[J].林业科学,2013,49(2):33-38,6.基金项目
国家林业局"948"引进项目(2009-4-24) （2009-4-24）
国家自然科学基金青年项目(30901156,31170619) （30901156,31170619）
江苏高校自然科学基金项目(09KJA220001) （09KJA220001）
江苏省高校优势学科建设工程资助项目. （）
