宁夏大学学报（自然科学版）2013，Vol.34Issue(1)：67-69,3.
从石蜡包埋的皮肤组织中提取核酸的两种脱蜡方法比较
Comparison of Two Methods for Dewaxing with Extracted Nucleic Acids From Paraffin-Embedded Skin Tissues
摘要
Abstract
To investigate a simple and practical method for extraction of nucleic acids from paraffin-embedded skin tissues, and evaluate the influences of dewaxing methods for DNA quality, twenty cases of pathology embedding psoriatic lesions tissues were selected and divided into xylene dewaxing group and improved TES dewaxing group. The results demonstrated that the ratio of OD260/ OD280 between 1. 6 and 1. 8 took 60% in DNA samples extracted by improved TES dewaxing method with water-bath, whereas it took 35% in DNA samples extracted by using xylene dewaxing method. , Considering the speed, practical and economic point of view, the improved TES dewaxing with water-bath followd by DNA extraction using phenol-chloroform is a method worthy of recommending for paraffin embedded skin tissues.关键词
石蜡包埋皮肤组织/TES溶液/DNA提取Key words
Paraffin-embedded skin tissues/ TES solution/ DNA extraction分类
医药卫生引用本文复制引用
刘新梅,张晓玲,赵宗峰,靳颖,吴卫东..从石蜡包埋的皮肤组织中提取核酸的两种脱蜡方法比较[J].宁夏大学学报（自然科学版）,2013,34(1):67-69,3.基金项目
国家自然科学基金资助项目(30640056) （30640056）
