山东医药2013，Vol.53Issue(12)：18-20,后插2,4.DOI:10.3969/j.issn.1002-266X.2013.12.006
软骨源性形态发生蛋白缓释系统对兔膝骨关节炎的影响
Study on the effects of cartilage-derived morphogenetic protein delivery system on rabbit knee osteoarthritis
摘要
Abstract
Objective Cartilage-derived morphogenetic protein-1 (CDMP-1) was prepared by applying hyaluronic acid (HA) , and then it was injected into the rabbit knee joint cavity, to observe the therapeutic effects on inhibiting osteoarthritis progress. Methods The model was made by injecting papain in the double knee joint cavities. After modeling for the first 3 and 6 weeks, all experimental groups were injected with 1 mL of rabbit articular saline (model group) , 1 mL CDMP-1 solution (CDMP-1-S group) , 1 mL PBS containing microspheres (HA-M group) and 1 mL microspheres with CDMP-1 (HA/CDMP-1-M group). At week 9, rats were killed. The untreated rats were used as blank control group. The general form and histopathology of the cartilage were evaluated; MMP-13, TIMP-1 mRNA expression changes were detected with RT-PCR. Results Compared with the control group, articular cartilage damage in model group was significantly increased; there was no significant articular cartilage damage mitigation in CDMP-1-S group and the HA-M group as compared with the model group. But, articular cartilage damage in HA/CDMP-1-M group as compared with the model group was significantly reduced. Conclusion Intra-articular injection with HA/CDMP-1-M can promote cartilage repairation and inhibit the progress of osteoarthritis.关键词
骨关节炎/软骨源性形态发生蛋白/缓释微球Key words
osteoarthritis/ cartilage-derived morphogenetic protein/ sustained-release microspheres分类
医药卫生引用本文复制引用
李成,赵硕,张振宇..软骨源性形态发生蛋白缓释系统对兔膝骨关节炎的影响[J].山东医药,2013,53(12):18-20,后插2,4.
