色谱2013，Vol.31Issue(4)：329-334,6.DOI:10.3724/SP.J.1123.2012.11009
一种混合模式反相强阳离子交换色谱固定相的制备及其保留机理的考察
Preparation and retention mechanism of a mixed-mode reversed-phase/strong-cationic-exchange chromatographic packing
摘要
Abstract
A simple and efficient method has been proposed for the preparation of octyl-sulfon-ic co-bonded silica (OSS) packing by the method of "mixed ligand". The resulting OSS packing was characterized by elemental analysis and ion-exchange capacity to prove the successful immobilization of octyl and sulfonic groups on the surface of silica gel. Then the retention mechanism of several basic analytes on the developed OSS phases was evaluated under the conditions of reversed-phase liquid chromatography (RPLC) mobile phase. The results indicated that the OSS stationary phases demonstrated a mixed-mode reversed-phase/strong-cationic-exchange (RP/SCX) retention mechanism and ion-exchange interaction maybe dominate the retention of the basic analytes. By changing the salt concentration of mobile phase, the one-site and two-site mixed-mode retention models of the several basic analytes on the OSS phases were obtained by investigating the logarithm and reciprocal relationships of retention factor and salt concentration. On the basis of the linear fitting of the two mathematical equations of the retention models, the experimental results demonstrated that the two-site model was more suitable for the description of the retention mechanism of the basic analytes on the OSS phases. Furthermore, the individual RP or SCX contribution to total retention was obtained according to the mathematical equations of the two-site retention mechanism, which can provide some valuable guidance for the separation of complex samples.关键词
反相强阳离子交换色谱固定相/混合模式色谱/保留机理/数学模型Key words
reversed-phase/strong-cationic-exchange (RP/SCX) chromatographic packing/ mixed-mode chromatography (MMC)/ retention mechanism/ mathematical model分类
化学化工引用本文复制引用
彭西甜,王珏,冯钰锜..一种混合模式反相强阳离子交换色谱固定相的制备及其保留机理的考察[J].色谱,2013,31(4):329-334,6.基金项目
国家自然科学基金项目(91019013,31070327). （91019013,31070327）
