西北师范大学学报（自然科学版）2013，Vol.49Issue(2)：75-79,5.
山西五台山不同海拔中国沙棘居群的遗传变异
ISSR analysis of genetic diversity of H rhamnoides ssp sinensis at different altitudes at Wutai Mountain in Shanxi Province
摘要
Abstract
In this paper, Hippophae rhamnoides ssp sinensis populations are collected from different altitudes at Wutai Mountain in Shanxi Province, and their genetic diversity and genetic differentiation are analysed by using the inter simple sequence repeat (ISSR) molecular technique. ISSR amplification of 60 individuals from 3 populations is conducted with 11 primers, and 162 polymorphic loci are detected from total 170 loci, with the percentage of polymorphic bands ( PPB) being 95.29%. The Shannon's information index(Ⅰ) and Nei's gene diversity (A) of H rhamnoides ssp sinensis from Wutai Mountain at species level are 0. 455 6 and 0. 301 1 respectively, indicating a high genetic diversity of H rhamnoides ssp sinensis at species level. High genetic diversity of H rhamnoides ssp sinensis at population level is also detected, with the mean values of PPB, I and h being 62.55%, 0.323 5 and 0.203 0 respectively. Population S15 and S16, which distributed at altitude of 203 3 m and 162 4 m respectively of Wutai Mountain, have higher genetic diversity than S14 at 121 4 m. POPGENE analysis shows the genetic differentiation coefficient (Gst) among the populations being 0.316 1. That mean among population component account for 31. 61% of the total variation, the gene flow among the populations is only 1. 081 8. The high genetic differentiation between populations implies the protection and utilization of resources should be considered in different areas' populations.关键词
中国沙棘/五台山/海拔/遗传变异Key words
H rhamnoides ssp sinensis/ Wutai Mountain/ altitude/ genetic diversity分类
农业科技引用本文复制引用
孙坤,王瑞雪,陈纹,侯勤正,李梅..山西五台山不同海拔中国沙棘居群的遗传变异[J].西北师范大学学报（自然科学版）,2013,49(2):75-79,5.基金项目
国家自然科学基金资助项目(31160046,30960029) （31160046,30960029）
