畜牧兽医学报2013，Vol.44Issue(3)：434-440,7.
新型猪源H1N1流感病毒的分离鉴定及在实验条件下气源性传播的特点
Isolation, Identification and Airborne Transmission Characteristics in the Experimental Conditions of Novel Swine-origin Influenza A (H1N1) Virus
摘要
Abstract
The novel swine-origin influenza A(H1N1) virus can cause respiratory infectious diseases in humans and pigs, it attracts wide attention and research since its worldwide outbreak in April 2009. This study researched the airborne spread characteristics of the virus. In January 2011, a swine-origin H1N1 epidemic emerged in eastern China, one novel swine-origin influenza A(H1N1) virus A/swine/Shandong/07/2011 was isolated from throat swab samples and lung collected from swine. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect viruses in air samples taken from inside and outside of piggeries; Aerosol transmission model were also established to analyze the airborne transmission characteristics in the experimental conditions of novel swine-origin influenza A (HIND virus. The detected rate of virus in piggeries air samples was 26. 10% and was shown to contain 3.14-5. 72 log10 copies·m-3 air of virus. The rate virus detected in 10 m downwind of piggeries air samples was 40. 70 % and was shown to contain 2. 24-3. 77 log10 copies·m-3 air of virus. In the model, this virus was capable of forming aerosols and infecting animals in aerosol exposure group by transfer of aerosols, although infection by aerosols was found to be less efficient than by direct contact. This study demonstrates that this SO 2009(H1N1)Ⅳ strain is able to be aerosolized by infected animals and to be transmitted to susceptible animals by airborne routes in the experimental conditions.关键词
新型猪源性H1N1流感病毒/荧光定量PCR/病毒气溶胶/气溶胶传播模型Key words
novel swine-origin influenza A(H1N1) virus/ quantitative reverse transcription polymerase chain reaction (RT-qPCR)/ viral aerosols/ aerosol transmission model分类
农业科技引用本文复制引用
李欣,张红娜,李欣贤,李晓霞,侯佩强,夏咸柱,苗增民,柴同杰..新型猪源H1N1流感病毒的分离鉴定及在实验条件下气源性传播的特点[J].畜牧兽医学报,2013,44(3):434-440,7.基金项目
国家环境保护环境微生物利用与安全控制重点实验2011年开放基金(MARC2011D061) （MARC2011D061）
国家科技支撑计划子课题,重大动物人兽共患病的防控技术研究及应用子课题(2010BAD04B000) （2010BAD04B000）
