畜牧兽医学报2013，Vol.44Issue(3)：465-474,10.
苦马豆内生真菌分离鉴定与多样性分析
Isolation and Identification of Endophytic Fungi in Sphaerophysa salsula and Its Diversity Analysis
摘要
Abstract
Endophytic fungi were isolated from Sphaerophysa salsula samples from Alashan of Inner Mongolia to investigate whether endophytic fungi exist in this plant and to assess the diversity and distribution of endophytic fungi. The optimal method for sterilization of plant materials surface with 75% ethanol and 2% NaClO was determined by the general method of endophytic fungus' isolation and purification, species and genus of endophytic fungus was identified by the traditional morphology and molecular biology. It was found that 75% enthanol for 30 s, 2% NaClO for 3. 5 min. and 75% enthanol for 50 s, 2% NaClO for 4 min. were more suitable for leaf and seed sterilization of Sphaerophysa salsula , respectively. Under the optimal surface sterilization conditions, leaf had a maximum isolation rates (41. 67%), followed with seed (37. 14%); and the species and the quantity ratio (38. 75%) of endophytic fungi isolated from seed was the highest, followed with stem (20. 00%). Eighty strains of endophytic fungi were isolated from 4 different tissues of Sphaerophysa salsula , which belonged to 10 genera, 6 families, 5 orders and 4 classes. Alternaria sp and Fusarium sp were the dominant species in this plant with the relative frequency 53. 75% and 21. 25%, respectively. There was an obviously difference in quantity, species and distribution of the endophytic fungi between different parts of Sphaerophysa salsula. Seed and leaf were the most vulnerable to infection and colonization by the endophytic fungi and the diversity was maximum in seed of Sphaerophysa salsula.关键词
苦马豆/内生真菌/分离鉴定/rDNA-ITS/多样性Key words
Sphaerophysa salsula/ endophytic fungi/ isolation and identification/ rDNA-ITS/ diversity分类
农业科技引用本文复制引用
周启武,赵宝玉,路浩,张潞,杨晓雯,张樑,温伟利..苦马豆内生真菌分离鉴定与多样性分析[J].畜牧兽医学报,2013,44(3):465-474,10.基金项目
国家自然科学基金(31201958) （31201958）
农业部"十二五"公益性行业(农业)科研专项(201203062) （农业）
中央高校基本科研业务费(QN2011108) （QN2011108）
